OsJAB1 Positively Regulates Ascorbate Biosynthesis and Negatively Regulates Salt Tolerance Due to Inhibiting Early-Stage Salt-Induced ROS Accumulation in Rice

Reactive oxygen species (ROS) play dual roles in plant stress response, but how plants modulate the dual roles of ROS in stress response is still obscure. OsJAB1 (JUN-activation-domain-binding protein 1) encodes the rice CSN5 (COP9 signalsome subunit 5). This study showed that, similar to the Arabid...

Full description

Saved in:
Bibliographic Details
Published in:Plants (Basel) 2023-11, Vol.12 (22), p.3859
Main Authors: Wang, Jiayi, Zhang, Chuanyu, Li, Hua, Xu, Yuejun, Zhang, Bo, Zheng, Fuyu, Zhao, Beiping, Zhang, Haiwen, Zhao, Hui, Liu, Baohai, Xiao, Minggang, Zhang, Zhijin
Format: Article
Language:English
Subjects:
Abiotic stress
Accumulation
Analysis
ascorbate
Ascorbic acid
Binding proteins
Biosynthesis
Enzymes
Gene expression
Genes
Genetic aspects
Genotype & phenotype
Growth
Homology
Identification and classification
Methods
OsJAB1
Plant growth
Plant stress
Polymerase chain reaction
Properties
Proteins
Reactive oxygen species
Rice
ROS
Salinity
Salinity tolerance
salt stress
Salt tolerance
Scavenging
Transgenic plants
Vitamin C
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Reactive oxygen species (ROS) play dual roles in plant stress response, but how plants modulate the dual roles of ROS in stress response is still obscure. OsJAB1 (JUN-activation-domain-binding protein 1) encodes the rice CSN5 (COP9 signalsome subunit 5). This study showed that, similar to the Arabidopsis homolog gene CSN5B, OsJAB1-overexpressing (driven by a CaMV 35S promoter) plants (OEs) impaired rice salt stress tolerance; in contrast, OsJAB1-inhibited-expression (using RNA-interfering technology) plants (RIs) enhanced rice salt stress tolerance. Differing from CSN5B that negatively regulated ascorbate (Asc) biosynthesis, Asc content increased in OEs and decreased in RIs. ROS analysis showed that RIs clearly increased, but OEs inhibited ROS accumulation at the early stage of salt treatment; in contrast, RIs clearly decreased, but OEs promoted ROS accumulation at the late stage of salt treatment. The qPCR revealed that OEs decreased but RIs enhanced the expressions of ROS-scavenging genes. This indicated that OsJAB1 negatively regulated rice salt stress tolerance by suppressing the expression of ROS-scavenging genes. This study provided new insights into the CSN5 homologous protein named OsJAB1 in rice, which developed different functions during long-term evolution. How OsJAB1 regulates the Asc biosynthesis that coordinates the balance between cell redox signaling and ROS scavenging needs to be investigated in the future.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants12223859