Development of a Polyphenol Oxidase Biosensor from Jenipapo Fruit Extract (Genipa americana L.) and Determination of Phenolic Compounds in Textile Industrial Effluents

• Published in: Biosensors (Basel) 2018-05, Vol.8 (2), p.47
• Main Authors: Antunes, Rafael Souza, Ferraz, Denes, Garcia, Luane Ferreira, Thomaz, Douglas Vieira, Luque, Rafael, Lobón, Germán Sanz, Gil, Eric de Souza, Lopes, Flávio Marques
• Format: Article
• Language: English
• Subjects: biosensor enzyme; Genipa americana L; phenolic compounds; polifenoloxidases
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios8020047

In this work, an innovative polyphenol oxidase biosensor was developed from Jenipapo ( L.) fruit and used to assess phenolic compounds in industrial effluent samples obtained from a textile industry located in Jaraguá-GO, Brasil. The biosensor was prepared and optimized according to: the proportion of crude vegetal extract, pH and overall voltammetric parameters for differential pulse voltammetry. The calibration curve presented a linear interval from 10 to 310 µM (r² = 0.9982) and a limit of detection of 7 µM. Biosensor stability was evaluated throughout 15 days, and it exhibited 88.22% of the initial response. The amount of catechol standard recovered post analysis varied between 87.50% and 96.00%. Moreover, the biosensor was able to detect phenolic compounds in a real sample, and the results were in accordance with standard spectrophotometric assays. Therefore, the innovatively-designed biosensor hereby proposed is a promising tool for phenolic compound detection and quantification when environmental contaminants are concerned.