Vacuolization in embryos on days 3 and 4 of in vitro development: Association with stimulation protocols, embryo development, chromosomal status, pregnancy and neonatal outcomes

Is vacuolization in embryos on Days 3 and 4 associated with parent-related factors, stimulation protocols, embryo development, embryo ploidy, pregnancy and neonatal outcomes? This is a retrospective cohort study that comprised 5,703 embryos from 611 patients who underwent preimplantation genetic tes...

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Published in:Frontiers in endocrinology (Lausanne) 2022-10, Vol.13, p.985741-985741
Main Authors: Chen, Longbin, Zhang, Shuoping, Gu, Yifan, Peng, Yangqin, Huang, Zenghui, Gong, Fei, Lin, Ge
Format: Article
Language:English
Subjects:
Blastocyst
clinical outcomes
Embryo Culture Techniques - methods
Embryo Implantation
embryo vacuoles
Embryonic Development - genetics
Endocrinology
euploidy
Female
Humans
neonatal outcomes
Pregnancy
Retrospective Studies
self-correction
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Summary:Is vacuolization in embryos on Days 3 and 4 associated with parent-related factors, stimulation protocols, embryo development, embryo ploidy, pregnancy and neonatal outcomes? This is a retrospective cohort study that comprised 5,703 embryos from 611 patients who underwent preimplantation genetic testing and time-lapse monitoring of their embryos from August 2017 to September 2021. Embryo vacuolization on Days 3 and 4 is associated with the LH level on the day of the hCG trigger and the number of retrieved oocytes. Compared to vacuole-negative embryos, the rates of blastocyst formation and good-blastocyst formation was significantly lower in vacuole-positive embryos. We observed no significant difference in the rates of euploidy, implantation, ongoing pregnancy, and live birth between vacuole-positive and vacuole-negative embryos. In vacuole-positive embryos, the embryos of which the vacuole-positive blastomeres were involved in embryo compaction exhibited significantly higher mosaicism rate compared with those of which the vacuole-positive blastomeres were not involved in embryo compaction. Vacuolization in embryos on Days 3 and 4 is associated with reduced blastocyst formation rate and high-quality blastocyst rate. Blastocysts had a low mosaicism rate if the vacuole-containing cells were rejected in compaction process, which supports the hypothesis that exclusion of abnormal blastomeres from compaction is a self-correction mechanism.
ISSN:1664-2392
1664-2392
DOI:10.3389/fendo.2022.985741