In vivo confocal microscopy evaluation of infiltrated immune cells in corneal stroma treated with cell therapy in advanced keratoconus

Purpose This study investigates immune cell (ICs) infiltration in advanced keratoconus patients undergoing autologous adipose-derived adult stem cell (ADASC) therapy with recellularized human donor corneal laminas (CL). Methods A prospective clinical trial included fourteen patients divided into thr...

Full description

Saved in:
Bibliographic Details
Published in:Journal of ophthalmic inflammation and infection 2024-01, Vol.14 (1), p.5-13, Article 5
Main Authors: El Zarif, Mona, Abdul Jawad, Karim, Alió, Jorge L., Makdissy, Nehman, De Miguel, María P.
Format: Article
Language:English
Subjects:
Adipose-derived adult stem cells
Anterior segment inflammation highlights update
Cell infiltration
Cell therapy
Cornea
Corneal laminas
Immune cells
Keratoconus
Medicine
Medicine & Public Health
Microscopy
Ophthalmology
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose This study investigates immune cell (ICs) infiltration in advanced keratoconus patients undergoing autologous adipose-derived adult stem cell (ADASC) therapy with recellularized human donor corneal laminas (CL). Methods A prospective clinical trial included fourteen patients divided into three groups: G-1, ADASCs; G-2, decellularized CL (dCL); and G-3, dCL recellularized with ADASCs (ADASCs-rCL). Infiltrated ICs were assessed using in vivo confocal microscopy (IVCM) at 1,3,6, and12 months post-transplant. Results Infiltrated ICs, encompassing granulocytes and agranulocytes, were observed across all groups, categorized by luminosity, structure, and area. Stromal ICs infiltration ranged from 1.19% to 6.62%, with a consistent increase in group-related cell density ( F  = 10.68, P  
ISSN:1869-5760
1869-5760
DOI:10.1186/s12348-024-00385-2