cis-Banglene, a bangle (Zingiber purpureum)-derived bioactive compound, promotes mitochondrial biogenesis and glucose uptake by activating the IL-6/AMPK signaling pathway in C2C12 skeletal muscle cells

• Published in: Journal of functional foods 2020-01, Vol.64, p.103632, Article 103632
• Main Authors: Norikura, Toshio, Kajiya, Shiori, Sugawara, Mami, Kubo, Miwa, Fukuyama, Yoshiyasu, Sato, Shin
• Format: Article
• Language: English
• Subjects: AMPK; Bangle; cis-banglene; Exercise mimetic; IL-6; STAT
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2019.103632

[Display omitted] •Effects of cis-banglene on skeletal muscle cells were examined.•cis-Banglene promoted glucose uptake and mitochondrial biogenesis like exercise.•cis-Banglene activated IL-6/AMPK signaling pathway like exercise.•cis-Banglene increased mRNA expression levels similar to exercise and AICAR. Recently, we reported that bangle intake promotes exercise-induced effects such as reducing insulin resistance and non-esterified fatty acid levels and ameliorating the loss of autophagy function in rats fed high-fat diets. In the present study, we examined the physiological effects of cis-banglene on C2C12 skeletal muscle cells. cis-Banglene activated AMPK, an exercise mimic target. It also promoted glucose uptake (2-NBDG uptake) and mitochondrial biogenesis (Rho 123 uptake and mtDNA copy number) as well as increased the mRNA expression (real-time PCR) and secretion of IL-6 (ELISA), a myokine produced by exercising skeletal muscles. Furthermore, cis-banglene significantly increased the mRNA expression levels of PPARγ, PGC-1α, LPL, CD36, and UCP3. These findings are consistent with previously reported changes in mRNA expression levels in the muscles during exercise. Thus, cis-banglene is a candidate exercise mimetic for increasing mitochondrial biogenesis and glucose uptake by activating the IL-6/AMPK signaling pathway.