Humidity-Dependent Bacterial Cells Functional Morphometry Investigations Using Atomic Force Microscope

The effect of a relative humidity (RH) in a range of 93–65% on morphological and elastic properties of Bacillus cereus and Escherichia coli cells was evaluated using atomic force microscopy. It is shown that gradual dehumidification of bacteria environment has no significant effect on cell dimension...

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Bibliographic Details
Published in:International Journal of Microbiology 2010-01, Vol.2010 (2010), p.255-259
Main Authors: Nikiyan, Hike, Vasilchenko, Alexey, Deryabin, Dmitry
Format: Article
Language:English
Subjects:
Bacillus cereus
Bacteria
Colleges & universities
E coli
Environmental conditions
Escherichia coli
Humidity
Methods
Microbiology
Microorganisms
Microscopy
Silicon nitride
Standard deviation
Standardization
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Summary:The effect of a relative humidity (RH) in a range of 93–65% on morphological and elastic properties of Bacillus cereus and Escherichia coli cells was evaluated using atomic force microscopy. It is shown that gradual dehumidification of bacteria environment has no significant effect on cell dimensional features and considerably decreases them only at 65% RH. The increasing of the bacteria cell wall roughness and elasticity occurs at the same time. Observed changes indicate that morphological properties of B. cereus are rather stable in wide range of relative humidity, whereas E. coli are more sensitive to drying, significantly increasing roughness and stiffness parameters at RH ≤ 84% RH. It is discussed the dependence of the response features on differences in cell wall structure of gram-positive and gram-negative bacterial cells.
ISSN:1687-918X
1687-9198
DOI:10.1155/2010/704170