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Development and characterization of microsatellite markers for Antennaria corymbosa (Asteraceae) and close relatives
Premise The genus Antennaria has a complex evolutionary history due to dioecism, excessive polyploidy, and the evolution of polyploid agamic complexes. We developed microsatellite markers from A. corymbosa to investigate genetic diversity and population genetic structure in Antennaria species. Metho...
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| Published in: | Applications in plant sciences 2019-06, Vol.7 (6), p.e11268-n/a |
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| container_issue | 6 |
| container_start_page | e11268 |
| container_title | Applications in plant sciences |
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| creator | Thapa, Ramhari Bayer, Randall J. Mandel, Jennifer R. |
| description | Premise
The genus Antennaria has a complex evolutionary history due to dioecism, excessive polyploidy, and the evolution of polyploid agamic complexes. We developed microsatellite markers from A. corymbosa to investigate genetic diversity and population genetic structure in Antennaria species.
Methods and Results
Twenty‐four novel microsatellite markers (16 nuclear and eight chloroplast) were developed from A. corymbosa using an enriched genomic library. Ten polymorphic nuclear markers were used to characterize genetic variation in five populations of A. corymbosa. One to four alleles were found per locus, and the expected heterozygosity and fixation index ranged from 0.00 to 0.675 and −0.033 to 0.610, respectively. We were also able to successfully amplify these markers in five additional Antennaria species.
Conclusions
These markers are promising tools to study the population genetics of sexual Antennaria species and to investigate interspecific gene flow, clonal diversity, and parentage of Antennaria polyploid agamic complexes. |
| doi_str_mv | 10.1002/aps3.11268 |
| format | article |
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The genus Antennaria has a complex evolutionary history due to dioecism, excessive polyploidy, and the evolution of polyploid agamic complexes. We developed microsatellite markers from A. corymbosa to investigate genetic diversity and population genetic structure in Antennaria species.
Methods and Results
Twenty‐four novel microsatellite markers (16 nuclear and eight chloroplast) were developed from A. corymbosa using an enriched genomic library. Ten polymorphic nuclear markers were used to characterize genetic variation in five populations of A. corymbosa. One to four alleles were found per locus, and the expected heterozygosity and fixation index ranged from 0.00 to 0.675 and −0.033 to 0.610, respectively. We were also able to successfully amplify these markers in five additional Antennaria species.
Conclusions
These markers are promising tools to study the population genetics of sexual Antennaria species and to investigate interspecific gene flow, clonal diversity, and parentage of Antennaria polyploid agamic complexes.</description><identifier>ISSN: 2168-0450</identifier><identifier>EISSN: 2168-0450</identifier><identifier>DOI: 10.1002/aps3.11268</identifier><identifier>PMID: 31236315</identifier><language>eng</language><publisher>United States: John Wiley & Sons, Inc</publisher><subject>Antennaria corymbosa ; Asteraceae ; Chloroplasts ; Deoxyribonucleic acid ; DNA ; Evolutionary genetics ; Gene flow ; Genetic diversity ; Genetic markers ; Genetic structure ; Genomes ; Heterozygosity ; Interspecific ; Library collections ; Microsatellites ; Plant sciences ; polyploid agamic complexes ; Polyploidy ; Population genetics ; Population studies ; Primer Note ; Primer Notes ; Species</subject><ispartof>Applications in plant sciences, 2019-06, Vol.7 (6), p.e11268-n/a</ispartof><rights>2019 Thapa et al. is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America</rights><rights>Copyright John Wiley & Sons, Inc. Jun 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5148-6b960719c6e6c47b443ef9823ae70ce42d834f85cfd6140b994acf176d96c6783</citedby><cites>FETCH-LOGICAL-c5148-6b960719c6e6c47b443ef9823ae70ce42d834f85cfd6140b994acf176d96c6783</cites><orcidid>0000-0003-3539-2991</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2247631423/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2247631423?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,11541,25731,27901,27902,36989,36990,44566,46027,46451,53766,53768,74869</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31236315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thapa, Ramhari</creatorcontrib><creatorcontrib>Bayer, Randall J.</creatorcontrib><creatorcontrib>Mandel, Jennifer R.</creatorcontrib><title>Development and characterization of microsatellite markers for Antennaria corymbosa (Asteraceae) and close relatives</title><title>Applications in plant sciences</title><addtitle>Appl Plant Sci</addtitle><description>Premise
The genus Antennaria has a complex evolutionary history due to dioecism, excessive polyploidy, and the evolution of polyploid agamic complexes. We developed microsatellite markers from A. corymbosa to investigate genetic diversity and population genetic structure in Antennaria species.
Methods and Results
Twenty‐four novel microsatellite markers (16 nuclear and eight chloroplast) were developed from A. corymbosa using an enriched genomic library. Ten polymorphic nuclear markers were used to characterize genetic variation in five populations of A. corymbosa. One to four alleles were found per locus, and the expected heterozygosity and fixation index ranged from 0.00 to 0.675 and −0.033 to 0.610, respectively. We were also able to successfully amplify these markers in five additional Antennaria species.
Conclusions
These markers are promising tools to study the population genetics of sexual Antennaria species and to investigate interspecific gene flow, clonal diversity, and parentage of Antennaria polyploid agamic complexes.</description><subject>Antennaria corymbosa</subject><subject>Asteraceae</subject><subject>Chloroplasts</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Evolutionary genetics</subject><subject>Gene flow</subject><subject>Genetic diversity</subject><subject>Genetic markers</subject><subject>Genetic structure</subject><subject>Genomes</subject><subject>Heterozygosity</subject><subject>Interspecific</subject><subject>Library collections</subject><subject>Microsatellites</subject><subject>Plant sciences</subject><subject>polyploid agamic complexes</subject><subject>Polyploidy</subject><subject>Population genetics</subject><subject>Population studies</subject><subject>Primer Note</subject><subject>Primer Notes</subject><subject>Species</subject><issn>2168-0450</issn><issn>2168-0450</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kl1rFDEUhoMottTe-AMk4E0tbM3XZJIbYalVCwUF9TqcyZxps85M1mR2Zf31Zju1tF6Ym4STh4fDOS8hLzk744yJt7DO8oxzoc0Tcii4NgumKvb0wfuAHOe8YuWYUrLqOTmQXEgteXVIpve4xT6uBxwnCmNL_Q0k8BOm8BumEEcaOzoEn2KGCfs-TEgHSD8wZdrFRJfjhOMIKQD1Me2GpnD0ZJmLADwCvpmlfcxIE_ZFucX8gjzroM94fHcfke8fLr6df1pcff54eb68WviKK7PQjdWs5tZr1F7VjVISO2uEBKyZRyVaI1VnKt-1mivWWKvAd7zWrdVe10YekcvZ20ZYuXUKpfOdixDcbSGmawdpCr5HZ1nlRVtMLdaq8tooyWujmxaFByt0cb2bXetNM2Dry7wS9I-kj3_GcOOu49bpyjBrqiI4uROk-HODeXJDyL6MFEaMm-yEUNoyZSUr6Ot_0FXcpLGMak_VZXNKyEKdztR-OTlhd98MZ26fDbfPhrvNRoFfPWz_Hv2bhALwGfgVetz9R-WWX77KWfoHokrFJw</recordid><startdate>201906</startdate><enddate>201906</enddate><creator>Thapa, Ramhari</creator><creator>Bayer, Randall J.</creator><creator>Mandel, Jennifer R.</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><general>Wiley</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3539-2991</orcidid></search><sort><creationdate>201906</creationdate><title>Development and characterization of microsatellite markers for Antennaria corymbosa (Asteraceae) and close relatives</title><author>Thapa, Ramhari ; Bayer, Randall J. ; Mandel, Jennifer R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5148-6b960719c6e6c47b443ef9823ae70ce42d834f85cfd6140b994acf176d96c6783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antennaria corymbosa</topic><topic>Asteraceae</topic><topic>Chloroplasts</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Evolutionary genetics</topic><topic>Gene flow</topic><topic>Genetic diversity</topic><topic>Genetic markers</topic><topic>Genetic structure</topic><topic>Genomes</topic><topic>Heterozygosity</topic><topic>Interspecific</topic><topic>Library collections</topic><topic>Microsatellites</topic><topic>Plant sciences</topic><topic>polyploid agamic complexes</topic><topic>Polyploidy</topic><topic>Population genetics</topic><topic>Population studies</topic><topic>Primer Note</topic><topic>Primer Notes</topic><topic>Species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thapa, Ramhari</creatorcontrib><creatorcontrib>Bayer, Randall J.</creatorcontrib><creatorcontrib>Mandel, Jennifer R.</creatorcontrib><collection>Wiley-Blackwell Open Access Collection</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>Biological Sciences</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Applications in plant sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thapa, Ramhari</au><au>Bayer, Randall J.</au><au>Mandel, Jennifer R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and characterization of microsatellite markers for Antennaria corymbosa (Asteraceae) and close relatives</atitle><jtitle>Applications in plant sciences</jtitle><addtitle>Appl Plant Sci</addtitle><date>2019-06</date><risdate>2019</risdate><volume>7</volume><issue>6</issue><spage>e11268</spage><epage>n/a</epage><pages>e11268-n/a</pages><issn>2168-0450</issn><eissn>2168-0450</eissn><abstract>Premise
The genus Antennaria has a complex evolutionary history due to dioecism, excessive polyploidy, and the evolution of polyploid agamic complexes. We developed microsatellite markers from A. corymbosa to investigate genetic diversity and population genetic structure in Antennaria species.
Methods and Results
Twenty‐four novel microsatellite markers (16 nuclear and eight chloroplast) were developed from A. corymbosa using an enriched genomic library. Ten polymorphic nuclear markers were used to characterize genetic variation in five populations of A. corymbosa. One to four alleles were found per locus, and the expected heterozygosity and fixation index ranged from 0.00 to 0.675 and −0.033 to 0.610, respectively. We were also able to successfully amplify these markers in five additional Antennaria species.
Conclusions
These markers are promising tools to study the population genetics of sexual Antennaria species and to investigate interspecific gene flow, clonal diversity, and parentage of Antennaria polyploid agamic complexes.</abstract><cop>United States</cop><pub>John Wiley & Sons, Inc</pub><pmid>31236315</pmid><doi>10.1002/aps3.11268</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-3539-2991</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Applications in plant sciences, 2019-06, Vol.7 (6), p.e11268-n/a |
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| source | Wiley-Blackwell Open Access Collection; Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central (PMC) |
| subjects | Antennaria corymbosa Asteraceae Chloroplasts Deoxyribonucleic acid DNA Evolutionary genetics Gene flow Genetic diversity Genetic markers Genetic structure Genomes Heterozygosity Interspecific Library collections Microsatellites Plant sciences polyploid agamic complexes Polyploidy Population genetics Population studies Primer Note Primer Notes Species |
| title | Development and characterization of microsatellite markers for Antennaria corymbosa (Asteraceae) and close relatives |
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