Preventive effects of selenium yeast, chromium picolinate, zinc sulfate and their combination on oxidative stress, inflammation, impaired angiogenesis and atherogenesis in myocardial infarction in rats

• Published in: Journal of pharmacy & pharmaceutical sciences 2013-01, Vol.16 (5), p.848-867
• Main Authors: Al-Rasheed, Nouf M, Attia, Hala A, Mohamed, Raessa A, Al-Rasheed, Nawal M, Al-Amin, Maha A
• Format: Article
• Language: English
• Subjects: Animals; Atherosclerosis - prevention & control; Cardiotonic Agents - blood; Cardiotonic Agents - pharmacokinetics; Cardiotonic Agents - therapeutic use; Drug Therapy, Combination; Dyslipidemias - drug therapy; Dyslipidemias - metabolism; Dyslipidemias - pathology; Inflammation - drug therapy; Inflammation - metabolism; Inflammation - pathology; Isoproterenol; Male; Myocardial Infarction - chemically induced; Myocardial Infarction - drug therapy; Myocardial Infarction - metabolism; Myocardial Infarction - pathology; Myocardium - metabolism; Myocardium - pathology; NADPH Oxidases - metabolism; Neovascularization, Physiologic - physiology; Oxidative Stress - drug effects; Picolinic Acids - blood; Picolinic Acids - pharmacokinetics; Picolinic Acids - therapeutic use; Rats; Rats, Wistar; Saccharomyces cerevisiae; Selenium - blood; Selenium - pharmacokinetics; Selenium - therapeutic use; Zinc Sulfate - blood; Zinc Sulfate - pharmacokinetics; Zinc Sulfate - therapeutic use
• ISSN: 1482-1826; 1482-1826
• DOI: 10.18433/j34c7n

Accumulating evidences suggest a critical role of trace metal dyshemostasis in oxidative stress and cardiac dysfunction after myocardial infarction (MI). This study investigated the cardioprotective effects of selenium yeast (Se), chromium picolinate Cr(pic)3, zinc sulfate (Zn) and their combination on isoproterenol (ISO)-induced MI. Rats were divided into six groups: normal control, ISO control, Se-pretreated (0.1 mg/kg), Cr(pic)3-pretreated (400 µg/kg), Zn-pretreated (30 mg/kg) and metal combination-pretreated groups. All metals were administered for 28 days and at the 27th day, MI was induced by subcutaneous injection of ISO (85 mg/kg) once for two consecutive days. ISO control group showed hyperlipidemia, elevation of cardiac biomarkers and lipid peroxidation and increased immunostaining of p47 phox NADPH oxidase subunit in addition to decreased levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Cardiac levels of tumor necrosis factor-α (TNF-α) were increased, while vascular endothelial growth factor (VEGF, the major angiogenic factor) was decreased. Pretreatment with Se normalized the cardiac enzymes, lipid peroxidation, GSH, SOD, CAT, GPx, TNF-α and VEGF (P