Effect of Sucrose on Growth and Stress Status of Castanea sativa x C. crenata Shoots Cultured in Liquid Medium

Current breeding programs aim to increase the number of ink-tolerant chestnut trees using vegetative propagation of selected genotypes. However, the commercial vegetative propagation of chestnut species is still a bottleneck for the forest industry, mainly due to problems in the rooting and acclimat...

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Published in:Plants (Basel) 2022-04, Vol.11 (7), p.965
Main Authors: Gago, Diego, Bernal, María Ángeles, Sánchez, Conchi, Aldrey, Anxela, Cuenca, Beatriz, Christie, Colin Bruce, Vidal, Nieves
Format: Article
Language:English
Subjects:
Acclimation
Acclimatization
Adaptation
Antioxidants
Biodiversity
Bioreactors
Carbon
Carbon dioxide
Castanea crenata
Castanea sativa
Chestnut
Climate change
continuous immersion
Explants
Genotypes
Humidity
Immersion
Laboratories
Light intensity
Luminous intensity
Micropropagation
Monosaccharides
Phenols
photoautotrophy
photosynthesis
Physiology
Plant propagation
Propagation
Propagules
Rooting
Shoots
Submerging
Sucrose
temporary immersion
Ventilation
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cited_by cdi_FETCH-LOGICAL-c484t-b008a02b5c92433d2273aea07b1cf5d8d2d22ac00a0c368ed29e2e62ee4afbb33
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container_issue 7
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container_title Plants (Basel)
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creator Gago, Diego
Bernal, María Ángeles
Sánchez, Conchi
Aldrey, Anxela
Cuenca, Beatriz
Christie, Colin Bruce
Vidal, Nieves
description Current breeding programs aim to increase the number of ink-tolerant chestnut trees using vegetative propagation of selected genotypes. However, the commercial vegetative propagation of chestnut species is still a bottleneck for the forest industry, mainly due to problems in the rooting and acclimation of propagules. This study aimed to explore the potential benefits of decreasing sucrose supplementation during chestnut micropropagation. Explants were cultured with high light intensity and CO -enriched air in temporary or continuous immersion bioreactors and with different sucrose supplementation to evaluate the impact of these treatments on growth, rooting and physiological status (monosaccharide content, soluble phenolics and antioxidant activity). The proliferation and rooting performance of shoots cultured by continuous immersion decreased sharply with sucrose concentrations lower than 1%, whereas shoots cultured by temporary immersion grew and rooted successfully with 0.5% sucrose. These results suggest this system is appropriate to culture chestnut with low sucrose concentration and to explore photoautotrophic propagation of this species.
doi_str_mv 10.3390/plants11070965
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subjects Acclimation
Acclimatization
Adaptation
Antioxidants
Biodiversity
Bioreactors
Carbon
Carbon dioxide
Castanea crenata
Castanea sativa
Chestnut
Climate change
continuous immersion
Explants
Genotypes
Humidity
Immersion
Laboratories
Light intensity
Luminous intensity
Micropropagation
Monosaccharides
Phenols
photoautotrophy
photosynthesis
Physiology
Plant propagation
Propagation
Propagules
Rooting
Shoots
Submerging
Sucrose
temporary immersion
Ventilation
title Effect of Sucrose on Growth and Stress Status of Castanea sativa x C. crenata Shoots Cultured in Liquid Medium
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