In Vitro Assessment of Organic and Residual Fractions of Nematicidal Culture Filtrates from Thirteen Tropical Trichoderma Strains and Metabolic Profiles of Most-Active

The nematicidal properties of species have potential for developing safer biocontrol agents. In the present study, 13 native strains from , (2 strains), (4), . , , and (4) with nematicidal activity were selected and cultured in potato dextrose broth to obtain a culture filtrate (CF) for each. Each C...

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Published in:Journal of fungi (Basel) 2022-01, Vol.8 (1), p.82
Main Authors: Moo-Koh, Felicia Amalia, Cristóbal-Alejo, Jairo, Andrés, María Fé, Martín, Jesús, Reyes, Fernando, Tun-Suárez, Jose María, Gamboa-Angulo, Marcela
Format: Article
Language:English
Subjects:
Acetic acid
Bioassays
Biological control
Chromatography
Dextrose
Ethyl acetate
ethyl acetate fraction
Fungi
HRMS dereplication
Humidity
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Meloidogyne incognita
Metabolites
Natural products
Nematodes
Scientific imaging
Sensors
Solvents
Trichoderma
Trichoderma ghanense
Trichoderma harzianum
Trichoderma koningiopsis
Trichoderma virens
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Summary:The nematicidal properties of species have potential for developing safer biocontrol agents. In the present study, 13 native strains from , (2 strains), (4), . , , and (4) with nematicidal activity were selected and cultured in potato dextrose broth to obtain a culture filtrate (CF) for each. Each CF was partitioned with ethyl acetate to obtain organic (EA) and residual filtrate (RF) fractions, which were then tested on second-stage juveniles (J2s) of the nematodes and in a microdilution assay. The most lethal strains were Th43-14, Th41-11, Th02-04, and Th32-09, which caused 51-100% mortality (%M) of J2s of both nematodes, mainly due to their RF fractions. Liquid chromatography-diode array detector-electrospray-high resolution mass spectrometry analysis of the most-active fractions revealed sesquiterpene and polyketide-like metabolites produced by the four active strains. These native strains have a high potential to develop safer natural products for the biocontrol of species.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof8010082