Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria

• Published in: BMC microbiology 2023-05, Vol.23 (1), p.142-142, Article 142
• Main Authors: Duche, Rachael T, Singh, Anamika, Wandhare, Arundhati Ganesh, Sangwan, Vikas, Sihag, Manvesh Kumar, Nwagu, Tochukwu N T, Panwar, Harsh, Ezeogu, Lewis I
• Format: Article
• Language: English
• Subjects: Aminoglycosides; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotics; Bacteria; beta-Lactams; Carbapenems; Cephalosporins; Ciprofloxacin; Composition; Drug resistance; Drug resistance in microorganisms; Drug Resistance, Bacterial - genetics; Fermented food; Fermented Foods; Food; Food sources; Functional foods; Functional foods & nutraceuticals; Gene amplification; Genetic aspects; Glycopeptides; Gram-positive bacteria; Health care; Humans; Identification; Lactic acid; Lactic acid bacteria; Lactobacillales - genetics; Lactobacilli; Lactobacillus; Maximum likelihood method; Methicillin; Microbial Sensitivity Tests; Milk; Multi drug resistant; Nigeria; Pathogens; Pharmaceuticals; Physiological aspects; Probiotics; Quinolones; Resistance genes; Software; Strains (organisms); Sulfonamides; Testing; β-Lactam antibiotics
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-023-02883-0

Probiotic lactobacilli are generally recognized as safe (GRAS) and are being used in several food and pharma formulations. However, growing concern of antibiotic resistance in bacterial strains of food origin and its possible transmission via functional foods is increasingly being emphasized. This study screened potential probiotic lactic acid bacteria (LAB) strains for their phenotypic and genotypic antibiotic resistance profiles. Susceptibility to different antibiotics was assayed by the Kirby Bauer standard disc diffusion protocol. Both conventional and SYBR-RTq-PCR were used for detection of resistance coding genes. A variable susceptibility pattern was documented against different antibiotic classes. LAB strains irrespective of origin displayed marked phenotypic resistance against cephalosporins, aminoglycosides, quinolones, glycopeptides; and methicillin among beta-lactams with few exceptions. In contrast, high sensitivity was recorded against macrolides, sulphonamides and carbapenems sub-group of beta-lactams with some variations. parC, associated with ciprofloxacin resistance was detected in 76.5% of the strains. Other prevalent resistant determinants observed were aac(6?)Ii (42.1%), ermB, ermC (29.4%), and tetM (20.5%). Six (?17.6%) of the isolates were free from genetic resistance determinants screened in this study. Study revealed presence of antibiotic resistance determinants among lactobacilli from both fermented foods and human sources.