Full-Length Transcriptome of Thalassiosira   weissflogii as a Reference Resource and Mining of Chitin-Related Genes

β-Chitin produced by diatoms is expected to have significant economic and ecological value due to its structure, which consists of parallel chains of chitin, its properties and the high abundance of diatoms. Nevertheless, few studies have functionally characterised chitin-related genes in diatoms ow...

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Bibliographic Details
Published in:Marine drugs 2021-07, Vol.19 (7), p.392
Main Authors: Cheng, Haomiao, Bowler, Chris, Xing, Xiaohui, Bulone, Vincent, Shao, Zhanru, Duan, Delin
Format: Article
Language:English
Subjects:
Alternative splicing
Animals
Bacillariophyceae
Biosynthetic Pathways
Cell walls
Chitin
Chitin - genetics
Chitosan
Communication
Data Mining
Diatoms
Diatoms - genetics
Economics
Enzymes
Exploitation
full-length transcriptome
Gene expression
Genes
Genomes
Length
Linkage analysis
Localization
Marine microorganisms
Metabolism
Non-coding RNA
Nucleotide sequence
PacBio sequencing
Proteins
Simple sequence repeats
Splicing
Thalassiosira weissflogii
Transcription
Transcription factors
Transcriptome
Transcriptomes
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Summary:β-Chitin produced by diatoms is expected to have significant economic and ecological value due to its structure, which consists of parallel chains of chitin, its properties and the high abundance of diatoms. Nevertheless, few studies have functionally characterised chitin-related genes in diatoms owing to the lack of omics-based information. In this study, we first compared the chitin content of three representative species. Cell wall glycosidic linkage analysis and chitin/chitosan staining assays showed that was an appropriate candidate chitin producer. A full-length (FL) transcriptome of was obtained via PacBio sequencing. In total, the FL transcriptome comprised 23,362 annotated unigenes, 710 long non-coding RNAs (lncRNAs), 363 transcription factors (TFs), 3113 alternative splicing (AS) events and 3295 simple sequence repeats (SSRs). More specifically, 234 genes related to chitin metabolism were identified and the complete biosynthetic pathways of chitin and chitosan were explored. The information presented here will facilitate molecular research and the exploitation of β-chitin-derived high-value enzymes and products.
ISSN:1660-3397
1660-3397
DOI:10.3390/md19070392