Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus

This study established a validated method for the quantitative and qualitative determination of eight signature compounds in . We used multiple-reaction monitoring scanning for quantification, and switched the electrospray ion source polarity between positive and negative modes in a single chromatog...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2020-08, Vol.25 (16), p.3632
Main Authors: Huang, Ho-Shin, Yu, Hsu-Sheng, Yen, Chia-Hung, Liaw, Ean-Tun
Format: Article
Language:English
Subjects:
Acids
Agricultural research
Antioxidants
Antioxidants - chemistry
Caffeic acid
Cardiovascular disease
Chlorogenic acid
Chromatography
Chromatography, High Pressure Liquid
Climate change
Correlation coefficient
Correlation coefficients
Eccoilopus formosanus
Ethanol
Ferulic acid
Food
Gallic acid
Gallic Acid - analysis
Ion sources
Ions
Limit of Detection
Mass spectrometry
Methods
Monitoring
multiple-reaction monitoring
phenolics
Phenols
Phenols - analysis
Plant Extracts - chemistry
Poaceae - chemistry
Poaceae - metabolism
Polarity
Polyphenols
Reproducibility of Results
Riboflavin
Scientific imaging
Tandem Mass Spectrometry - methods
Thiamine
Thiamine - analysis
Vitamin B
Vitamins
Vitamins - analysis
Water - chemistry
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Summary:This study established a validated method for the quantitative and qualitative determination of eight signature compounds in . We used multiple-reaction monitoring scanning for quantification, and switched the electrospray ion source polarity between positive and negative modes in a single chromatographic run. The precursor-to-product ion transitions were 355/163, 181/163, 265/122, 269/117, 170/152, 377.2/180.7, 169/124.8 and 193/134 for chlorogenic acid, caffeic acid, thiamine, apigenin, pyridoxamin, riboflavin, gallic acid and ferulic acid, respectively. The developed method was also validated for accuracy, precision and limit of quantification. In this method, eight compounds were quantified with correlation coefficients of greater than 0.995. A high recovery (81.5-94.1%) and good reproducibility was obtained for five phenolics and three vitamins with the relative standard deviation, ranging from 1.2 to 3.5%. This method may be applied to the determination of both phenolics and water-soluble vitamins in cereal grain. The results may suggest that the extract of could be a good source of bioactive phytochemicals.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules25163632