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Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus
This study established a validated method for the quantitative and qualitative determination of eight signature compounds in . We used multiple-reaction monitoring scanning for quantification, and switched the electrospray ion source polarity between positive and negative modes in a single chromatog...
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| Published in: | Molecules (Basel, Switzerland) Switzerland), 2020-08, Vol.25 (16), p.3632 |
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| Main Authors: | , , , |
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| container_issue | 16 |
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| creator | Huang, Ho-Shin Yu, Hsu-Sheng Yen, Chia-Hung Liaw, Ean-Tun |
| description | This study established a validated method for the quantitative and qualitative determination of eight signature compounds in
. We used multiple-reaction monitoring scanning for quantification, and switched the electrospray ion source polarity between positive and negative modes in a single chromatographic run. The precursor-to-product ion transitions were
355/163,
181/163,
265/122,
269/117,
170/152,
377.2/180.7,
169/124.8 and
193/134 for chlorogenic acid, caffeic acid, thiamine, apigenin, pyridoxamin, riboflavin, gallic acid and ferulic acid, respectively. The developed method was also validated for accuracy, precision and limit of quantification. In this method, eight compounds were quantified with correlation coefficients of greater than 0.995. A high recovery (81.5-94.1%) and good reproducibility was obtained for five phenolics and three vitamins with the relative standard deviation, ranging from 1.2 to 3.5%. This method may be applied to the determination of both phenolics and water-soluble vitamins in cereal grain. The results may suggest that the extract of
could be a good source of bioactive phytochemicals. |
| doi_str_mv | 10.3390/molecules25163632 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_90eef93fd8f6470db83d6c6d20aef82b</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_90eef93fd8f6470db83d6c6d20aef82b</doaj_id><sourcerecordid>2434056914</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4082-7f93378b2e8ecb60e2824452b62179a12368ced52c5cec38877b22b39cc403c53</originalsourceid><addsrcrecordid>eNplkktv1DAURi0EomXgB7CpLLFhE_ArjrOphNoClToCQYGl5Tg3Mx459tROkNjyy_HMlKqFlS3f8x2_LkIvKXnDeUvejtGDnT1kVlPJJWeP0DEVjFSciPbxvfkRepbzhhBGBa2foiPOGlUT0Ryj38vZT27rofoCxk4uBryMwU0xubDC1yb0MOKlyRkvYVrHHg8x4XOYII0umD0fB_x5DSF6ZzMuAfzDlHL1Nfq584C_u8kUNmMX8IW10fm4nfPOM8ZswpyfoyeD8Rle3I4L9O39xfXZx-rq04fLs3dXlRVEsaoZWs4b1TFQYDtJgCkmRM06yWjTGsq4VBb6mtnaguVKNU3HWMdbW_Lc1nyBLg_ePpqN3iY3mvRLR-P0fiGmlTZpctaDbglA2W7o1SBFQ_pO8V5a2TNiYFBFukCnB9d27kboLYQpGf9A-rAS3Fqv4k_dCCnKjxTB61tBijcz5EmPLlvw3gSIc9ZMcEFq2dId-uofdBPnFMpT7SkhOCWkUPRA2RRzTjDcHYYSvesW_V-3lMzJ_VvcJf62B_8D6aO_nA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2434443100</pqid></control><display><type>article</type><title>Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Huang, Ho-Shin ; Yu, Hsu-Sheng ; Yen, Chia-Hung ; Liaw, Ean-Tun</creator><creatorcontrib>Huang, Ho-Shin ; Yu, Hsu-Sheng ; Yen, Chia-Hung ; Liaw, Ean-Tun</creatorcontrib><description>This study established a validated method for the quantitative and qualitative determination of eight signature compounds in
. We used multiple-reaction monitoring scanning for quantification, and switched the electrospray ion source polarity between positive and negative modes in a single chromatographic run. The precursor-to-product ion transitions were
355/163,
181/163,
265/122,
269/117,
170/152,
377.2/180.7,
169/124.8 and
193/134 for chlorogenic acid, caffeic acid, thiamine, apigenin, pyridoxamin, riboflavin, gallic acid and ferulic acid, respectively. The developed method was also validated for accuracy, precision and limit of quantification. In this method, eight compounds were quantified with correlation coefficients of greater than 0.995. A high recovery (81.5-94.1%) and good reproducibility was obtained for five phenolics and three vitamins with the relative standard deviation, ranging from 1.2 to 3.5%. This method may be applied to the determination of both phenolics and water-soluble vitamins in cereal grain. The results may suggest that the extract of
could be a good source of bioactive phytochemicals.</description><identifier>ISSN: 1420-3049</identifier><identifier>EISSN: 1420-3049</identifier><identifier>DOI: 10.3390/molecules25163632</identifier><identifier>PMID: 32785047</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acids ; Agricultural research ; Antioxidants ; Antioxidants - chemistry ; Caffeic acid ; Cardiovascular disease ; Chlorogenic acid ; Chromatography ; Chromatography, High Pressure Liquid ; Climate change ; Correlation coefficient ; Correlation coefficients ; Eccoilopus formosanus ; Ethanol ; Ferulic acid ; Food ; Gallic acid ; Gallic Acid - analysis ; Ion sources ; Ions ; Limit of Detection ; Mass spectrometry ; Methods ; Monitoring ; multiple-reaction monitoring ; phenolics ; Phenols ; Phenols - analysis ; Plant Extracts - chemistry ; Poaceae - chemistry ; Poaceae - metabolism ; Polarity ; Polyphenols ; Reproducibility of Results ; Riboflavin ; Scientific imaging ; Tandem Mass Spectrometry - methods ; Thiamine ; Thiamine - analysis ; Vitamin B ; Vitamins ; Vitamins - analysis ; Water - chemistry</subject><ispartof>Molecules (Basel, Switzerland), 2020-08, Vol.25 (16), p.3632</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4082-7f93378b2e8ecb60e2824452b62179a12368ced52c5cec38877b22b39cc403c53</citedby><cites>FETCH-LOGICAL-c4082-7f93378b2e8ecb60e2824452b62179a12368ced52c5cec38877b22b39cc403c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2434443100/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2434443100?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25732,27903,27904,36991,36992,44569,53769,53771,74872</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32785047$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Ho-Shin</creatorcontrib><creatorcontrib>Yu, Hsu-Sheng</creatorcontrib><creatorcontrib>Yen, Chia-Hung</creatorcontrib><creatorcontrib>Liaw, Ean-Tun</creatorcontrib><title>Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus</title><title>Molecules (Basel, Switzerland)</title><addtitle>Molecules</addtitle><description>This study established a validated method for the quantitative and qualitative determination of eight signature compounds in
. We used multiple-reaction monitoring scanning for quantification, and switched the electrospray ion source polarity between positive and negative modes in a single chromatographic run. The precursor-to-product ion transitions were
355/163,
181/163,
265/122,
269/117,
170/152,
377.2/180.7,
169/124.8 and
193/134 for chlorogenic acid, caffeic acid, thiamine, apigenin, pyridoxamin, riboflavin, gallic acid and ferulic acid, respectively. The developed method was also validated for accuracy, precision and limit of quantification. In this method, eight compounds were quantified with correlation coefficients of greater than 0.995. A high recovery (81.5-94.1%) and good reproducibility was obtained for five phenolics and three vitamins with the relative standard deviation, ranging from 1.2 to 3.5%. This method may be applied to the determination of both phenolics and water-soluble vitamins in cereal grain. The results may suggest that the extract of
could be a good source of bioactive phytochemicals.</description><subject>Acids</subject><subject>Agricultural research</subject><subject>Antioxidants</subject><subject>Antioxidants - chemistry</subject><subject>Caffeic acid</subject><subject>Cardiovascular disease</subject><subject>Chlorogenic acid</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Climate change</subject><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Eccoilopus formosanus</subject><subject>Ethanol</subject><subject>Ferulic acid</subject><subject>Food</subject><subject>Gallic acid</subject><subject>Gallic Acid - analysis</subject><subject>Ion sources</subject><subject>Ions</subject><subject>Limit of Detection</subject><subject>Mass spectrometry</subject><subject>Methods</subject><subject>Monitoring</subject><subject>multiple-reaction monitoring</subject><subject>phenolics</subject><subject>Phenols</subject><subject>Phenols - analysis</subject><subject>Plant Extracts - chemistry</subject><subject>Poaceae - chemistry</subject><subject>Poaceae - metabolism</subject><subject>Polarity</subject><subject>Polyphenols</subject><subject>Reproducibility of Results</subject><subject>Riboflavin</subject><subject>Scientific imaging</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Thiamine</subject><subject>Thiamine - analysis</subject><subject>Vitamin B</subject><subject>Vitamins</subject><subject>Vitamins - analysis</subject><subject>Water - chemistry</subject><issn>1420-3049</issn><issn>1420-3049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNplkktv1DAURi0EomXgB7CpLLFhE_ArjrOphNoClToCQYGl5Tg3Mx459tROkNjyy_HMlKqFlS3f8x2_LkIvKXnDeUvejtGDnT1kVlPJJWeP0DEVjFSciPbxvfkRepbzhhBGBa2foiPOGlUT0Ryj38vZT27rofoCxk4uBryMwU0xubDC1yb0MOKlyRkvYVrHHg8x4XOYII0umD0fB_x5DSF6ZzMuAfzDlHL1Nfq584C_u8kUNmMX8IW10fm4nfPOM8ZswpyfoyeD8Rle3I4L9O39xfXZx-rq04fLs3dXlRVEsaoZWs4b1TFQYDtJgCkmRM06yWjTGsq4VBb6mtnaguVKNU3HWMdbW_Lc1nyBLg_ePpqN3iY3mvRLR-P0fiGmlTZpctaDbglA2W7o1SBFQ_pO8V5a2TNiYFBFukCnB9d27kboLYQpGf9A-rAS3Fqv4k_dCCnKjxTB61tBijcz5EmPLlvw3gSIc9ZMcEFq2dId-uofdBPnFMpT7SkhOCWkUPRA2RRzTjDcHYYSvesW_V-3lMzJ_VvcJf62B_8D6aO_nA</recordid><startdate>20200810</startdate><enddate>20200810</enddate><creator>Huang, Ho-Shin</creator><creator>Yu, Hsu-Sheng</creator><creator>Yen, Chia-Hung</creator><creator>Liaw, Ean-Tun</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20200810</creationdate><title>Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus</title><author>Huang, Ho-Shin ; Yu, Hsu-Sheng ; Yen, Chia-Hung ; Liaw, Ean-Tun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4082-7f93378b2e8ecb60e2824452b62179a12368ced52c5cec38877b22b39cc403c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acids</topic><topic>Agricultural research</topic><topic>Antioxidants</topic><topic>Antioxidants - chemistry</topic><topic>Caffeic acid</topic><topic>Cardiovascular disease</topic><topic>Chlorogenic acid</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Climate change</topic><topic>Correlation coefficient</topic><topic>Correlation coefficients</topic><topic>Eccoilopus formosanus</topic><topic>Ethanol</topic><topic>Ferulic acid</topic><topic>Food</topic><topic>Gallic acid</topic><topic>Gallic Acid - analysis</topic><topic>Ion sources</topic><topic>Ions</topic><topic>Limit of Detection</topic><topic>Mass spectrometry</topic><topic>Methods</topic><topic>Monitoring</topic><topic>multiple-reaction monitoring</topic><topic>phenolics</topic><topic>Phenols</topic><topic>Phenols - analysis</topic><topic>Plant Extracts - chemistry</topic><topic>Poaceae - chemistry</topic><topic>Poaceae - metabolism</topic><topic>Polarity</topic><topic>Polyphenols</topic><topic>Reproducibility of Results</topic><topic>Riboflavin</topic><topic>Scientific imaging</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Thiamine</topic><topic>Thiamine - analysis</topic><topic>Vitamin B</topic><topic>Vitamins</topic><topic>Vitamins - analysis</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Ho-Shin</creatorcontrib><creatorcontrib>Yu, Hsu-Sheng</creatorcontrib><creatorcontrib>Yen, Chia-Hung</creatorcontrib><creatorcontrib>Liaw, Ean-Tun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Molecules (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Ho-Shin</au><au>Yu, Hsu-Sheng</au><au>Yen, Chia-Hung</au><au>Liaw, Ean-Tun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus</atitle><jtitle>Molecules (Basel, Switzerland)</jtitle><addtitle>Molecules</addtitle><date>2020-08-10</date><risdate>2020</risdate><volume>25</volume><issue>16</issue><spage>3632</spage><pages>3632-</pages><issn>1420-3049</issn><eissn>1420-3049</eissn><abstract>This study established a validated method for the quantitative and qualitative determination of eight signature compounds in
. We used multiple-reaction monitoring scanning for quantification, and switched the electrospray ion source polarity between positive and negative modes in a single chromatographic run. The precursor-to-product ion transitions were
355/163,
181/163,
265/122,
269/117,
170/152,
377.2/180.7,
169/124.8 and
193/134 for chlorogenic acid, caffeic acid, thiamine, apigenin, pyridoxamin, riboflavin, gallic acid and ferulic acid, respectively. The developed method was also validated for accuracy, precision and limit of quantification. In this method, eight compounds were quantified with correlation coefficients of greater than 0.995. A high recovery (81.5-94.1%) and good reproducibility was obtained for five phenolics and three vitamins with the relative standard deviation, ranging from 1.2 to 3.5%. This method may be applied to the determination of both phenolics and water-soluble vitamins in cereal grain. The results may suggest that the extract of
could be a good source of bioactive phytochemicals.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>32785047</pmid><doi>10.3390/molecules25163632</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Agricultural research Antioxidants Antioxidants - chemistry Caffeic acid Cardiovascular disease Chlorogenic acid Chromatography Chromatography, High Pressure Liquid Climate change Correlation coefficient Correlation coefficients Eccoilopus formosanus Ethanol Ferulic acid Food Gallic acid Gallic Acid - analysis Ion sources Ions Limit of Detection Mass spectrometry Methods Monitoring multiple-reaction monitoring phenolics Phenols Phenols - analysis Plant Extracts - chemistry Poaceae - chemistry Poaceae - metabolism Polarity Polyphenols Reproducibility of Results Riboflavin Scientific imaging Tandem Mass Spectrometry - methods Thiamine Thiamine - analysis Vitamin B Vitamins Vitamins - analysis Water - chemistry |
| title | Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus |
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