Differentiation of wild-type varicella-zoster strains from India and the Oka vaccine strain using a VZV open reading frame - 62 based PCR-RFLP technique

Since the introduction of varicella vaccination in India, surveillance of circulating VZV strains has gained significance. Differentiating wild-type VZV strains from the Oka vaccine strain can be achieved only by molecular genotyping methods. The development of PCR methods for VZV strain differentia...

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Bibliographic Details
Published in:The Brazilian journal of infectious diseases 2008-08, Vol.12 (4), p.313-315
Main Authors: Kaushik, Karishma S, Lahiri, Kunal K, Kapila, Ketoki, Kumar, Satish, Gupta, Rajiv M, Karade, Santosh
Format: Article
Language:English
Subjects:
Chickenpox
Chickenpox - immunology
Chickenpox - virology
Chickenpox vaccine
Chickenpox Vaccine - genetics
Chickenpox Vaccine - immunology
DNA, Viral - analysis
Genetic aspects
Genotype
Health aspects
Herpes Zoster - immunology
Herpes Zoster - virology
Herpesvirus 3, Human - classification
Herpesvirus 3, Human - genetics
Herpesvirus 3, Human - immunology
Humans
India
INFECTIOUS DISEASES
OKA strain
Open Reading Frames - genetics
ORF 62
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Prevention
Restriction fragment length polymorphism analysis
Varicella-zoster virus
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Summary:Since the introduction of varicella vaccination in India, surveillance of circulating VZV strains has gained significance. Differentiating wild-type VZV strains from the Oka vaccine strain can be achieved only by molecular genotyping methods. The development of PCR methods for VZV strain differentiation has been hampered by the fact that the VZV genome is highly conserved. We used VZV ORF 62 PCR-RFLP analysis to identify and differentiate wild-type VZV strains in India from the Oka vaccine strain. Digestion of VZV ORF 62 amplicons with SmaI, enabled accurate strain differentiation; the Oka strain was positive for three SmaI sites, compared to two SmaI sites in the wild-type VZV strains that we tested.
ISSN:1413-8670
1678-4391
1678-4391
1413-8670
DOI:10.1590/S1413-86702008000400011