Development of antagonistic yeasts for controlling black mold disease of onion

Background The present study aimed to examine the biocontrol efficacy of 28 yeast isolates against Aspergillus niger , the common pathogen of onion black mold disease. The antagonistic potential of yeast isolates against A. niger growth was investigated using a dual culture technique. Results Five y...

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Bibliographic Details
Published in:Egyptian journal of biological pest control 2023-12, Vol.33 (1), p.17-7, Article 17
Main Authors: Bagy, Hadeel M. M. Kalil, Abo-Elyousr, Kamal A. M., Hesham, Abd El-Latif, Sallam, Nashwa M. A.
Format: Article
Language:English
Subjects:
Antagonistic yeasts
Aspergillus niger
Biological control
Biomedical and Life Sciences
Cultural filtrate
Culture
Culture techniques
Disease control
Formulations
Life Sciences
Mold
Onion
Onions
Pathogens
Shelf life
Yeast
Yeasts
Zoology
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Summary:Background The present study aimed to examine the biocontrol efficacy of 28 yeast isolates against Aspergillus niger , the common pathogen of onion black mold disease. The antagonistic potential of yeast isolates against A. niger growth was investigated using a dual culture technique. Results Five yeast isolates, including 8 and 11, showed the highest inhibition effect on the mycelial growth of A. niger . Molecular analysis using 16S RNA identified strains 8 (AUN-AH14) and 11 (AUN-AH23) as Galactomyces geotrichum (JQ713185.1) and G. geotrichum (DQ849321.1), respectively. The culture filtrates of AUN-AH14 and AUN-AH23 at percentage concentrations of 20, 40, 60, and 80 were tested against the growth of A. niger . As a result, the lowest dry weight of the pathogen was obtained with culture filtrates of the two strains at concentrations of 60 and 80%.. Treatment of onion bulbs with formulation of antagonistic yeast isolates AUN-AH14 and AUN-AH23 and their corresponding cultural filtrates at 80% concentration significantly reduced the severity of black mold disease relative to the controls. Conclusions The most significant reduction in black mold severity was proved in yeast formulations of AUN-AH14 and AUN-AH23, followed by their cultural filtrates (CF-AUN-AH14 and CF-AUN-AH23). The shelf life of formulated yeast isolates began to gradually deteriorate after 5 months of storage at 4 °C.
ISSN:2536-9342
1110-1768
2536-9342
DOI:10.1186/s41938-023-00664-5