In vitro anti- biofilm and anti-bacterial activity of Sesbania grandiflora extract against Staphylococcus aureus

The main objective of this research is to investigate the anti-biofilm and anti-bacterial activity of ( extract were prepared and analyzed with UV -Vis spectroscopy, Fourier transform infrared spectroscopy, Dynamic light scattering. Biofilm forming pathogens were identified by congo-red assay. Quant...

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Bibliographic Details
Published in:Biochemistry and biophysics reports 2017-12, Vol.12 (C), p.193-197
Main Authors: Gandhi, Arumugam Dhanesh, Vizhi, Dhandapani Kayal, Lavanya, Kubendiran, Kalpana, V N, Devi Rajeswari, V, Babujanarthanam, Ranganathan
Format: Article
Language:English
Subjects:
Congo-red assay
Extra polymorphic substances (EPS)
FTIR
Sesbania grandiflora
Staphylococcus aureus
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Summary:The main objective of this research is to investigate the anti-biofilm and anti-bacterial activity of ( extract were prepared and analyzed with UV -Vis spectroscopy, Fourier transform infrared spectroscopy, Dynamic light scattering. Biofilm forming pathogens were identified by congo-red assay. Quantification of Extracellular polymeric substance (EPS) particularly protein and carbohydrate were calculated. The efficacy of the herbal extract and its inhibition against the pathogenic strain of was also evaluated. The gradual decrease or disappearance of peaks reveals the reduction of protein and carbohydrate content in the EPS of when treated with . The antibacterial activity of extract against the bacterial strain showed that the extract were more active against the strain. To conclude, anti-biofilm and antibacterial efficacy of plays a vital role over biofilm producing pathogens and act as a good source for controlling the microbial population.
ISSN:2405-5808
2405-5808
DOI:10.1016/j.bbrep.2017.10.004