Developmental competence of different quality bovine oocytes retrieved through ovum pick-up following in vitro maturation and fertilization

In the present study, oocytes retrieved from cross bred Karan Fries cows by ovum pick-up technique were graded into Group 1 and Group 2, based on the morphological appearance of the individual cumulus–oocyte complexes (COCs). To analyze whether the developmental potential of the COCs bears a relatio...

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Bibliographic Details
Published in:Animal (Cambridge, England) England), 2015-12, Vol.9 (12), p.1979-1985
Main Authors: Saini, N., Singh, M. K., Shah, S. M., Singh, K. P., Kaushik, R., Manik, R. S., Singla, S. K., Palta, P., Chauhan, M. S.
Format: Article
Language:English
Subjects:
Animals
Apoptosis
blastocyst
Blastocyst - physiology
bovine
Cattle - embryology
Cattle - genetics
Cattle - physiology
cows
developmental competence
embryogenesis
Embryonic Development
Female
Fertilization in Vitro - veterinary
gene expression
Gene Expression Regulation, Developmental
genes
germinal vesicle
in vitro fertilization
Karan fries
Oocytes - physiology
ovum pick-up
Physiology and functional biology of systems
Pregnancy
Research Article
zygote
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Summary:In the present study, oocytes retrieved from cross bred Karan Fries cows by ovum pick-up technique were graded into Group 1 and Group 2, based on the morphological appearance of the individual cumulus–oocyte complexes (COCs). To analyze whether the developmental potential of the COCs bears a relation to morphological appearance, relative expression of a panel of genes associated with; (a) cumulus–oocyte interaction (Cx43, Cx37, GDF9 and BMP15), (b) fertilization (ZP2 and ZP3), (c) embryonic development (HSF1, ZAR1 and bFGF) and (d) apoptosis and survival (BAX, BID and BCL-XL, MCL-1, respectively) was studied at two stages: germinal vesicle (GV) stage and after in vitro maturation. The competence was further corroborated by evaluating the embryonic progression of the presumed zygotes obtained from fertilization of the graded COCs. The gene expression profile and development rate in pooled A and B grade (Group 1) COCs and pooled C and D grade (Group 2) COCs were determined and compared according to the original grades. The results of the study demonstrated that the morphologically characterized Group 2 COCs showed significantly (P
ISSN:1751-7311
1751-732X
1751-732X
DOI:10.1017/S1751731115001226