Esrrb Regulates Specific Feed-Forward Loops to Transit From Pluripotency Into Early Stages of Differentiation

Characterization of pluripotent states, in which cells can both self-renew or differentiate, with the irreversible loss of pluripotency, are important research areas in developmental biology. Although microRNAs (miRNAs) have been shown to play a relevant role in cellular differentiation, the role of...

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Bibliographic Details
Published in:Frontiers in cell and developmental biology 2022-05, Vol.10, p.820255-820255
Main Authors: Mazloom, Amin R, Xu, Huilei, Reig-Palou, Jaume, Vasileva, Ana, Román, Angel-Carlos, Mulero-Navarro, Sonia, Lemischka, Ihor R, Sevilla, Ana
Format: Article
Language:English
Subjects:
Cell and Developmental Biology
estrogen related receptor beta
feed-forward loops
MicroRNAs
mouse embryonic stem cells
network dynamic analysis
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Summary:Characterization of pluripotent states, in which cells can both self-renew or differentiate, with the irreversible loss of pluripotency, are important research areas in developmental biology. Although microRNAs (miRNAs) have been shown to play a relevant role in cellular differentiation, the role of miRNAs integrated into gene regulatory networks and its dynamic changes during these early stages of embryonic stem cell (ESC) differentiation remain elusive. Here we describe the dynamic transcriptional regulatory circuitry of stem cells that incorporate protein-coding and miRNA genes based on miRNA array expression and quantitative sequencing of short transcripts upon the downregulation of the Estrogen Related Receptor Beta (Esrrb). The data reveals how Esrrb, a key stem cell transcription factor, regulates a specific stem cell miRNA expression program and integrates dynamic changes of feed-forward loops contributing to the early stages of cell differentiation upon its downregulation. Together these findings provide new insights on the architecture of the combined transcriptional post-transcriptional regulatory network in embryonic stem cells.
ISSN:2296-634X
2296-634X
DOI:10.3389/fcell.2022.820255