Polyphenolics with Strong Antioxidant Activity from Acacia nilotica Ameliorate Some Biochemical Signs of Arsenic-Induced Neurotoxicity and Oxidative Stress in Mice

• Published in: Molecules (Basel, Switzerland) Switzerland), 2022-02, Vol.27 (3), p.1037
• Main Authors: Foyzun, Tahira, Mahmud, Abdullah Al, Ahammed, Md Salim, Manik, Md Imran Nur, Hasan, Md Kamrul, Islam, K M Monirul, Lopa, Simin Sobnom, Al-Amin, Md Yusuf, Biswas, Kushal, Afrin, Mst Rejina, Alam, Ahm Khurshid, Sadik, Golam
• Format: Article
• Language: English
• Subjects: Acacia - chemistry; Acacia nilotica; Acetylcholinesterase; Acids; Aluminum; Animals; Antioxidants; Antioxidants - chemistry; Antioxidants - therapeutic use; Arsenic; Arsenic - toxicity; Arsenic Poisoning - drug therapy; Arsenic Poisoning - metabolism; biomarker; Catechin; Chloride; Column chromatography; Depletion; Drinking water; Flavonoids; Free radicals; Gallic acid; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Male; Memory; Mice; Neurotoxicity; Oxidative stress; Oxidative Stress - drug effects; Peroxidation; Phenolic compounds; phenolics; Phenols; Polyphenols; Polyphenols - chemistry; Polyphenols - therapeutic use; Potassium; Scavenging
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27031037

Neurotoxicity is a serious health problem of patients chronically exposed to arsenic. There is no specific treatment of this problem. Oxidative stress has been implicated in the pathological process of neurotoxicity. Polyphenolics have proven antioxidant activity, thereby offering protection against oxidative stress. In this study, we have isolated the polyphenolics from and investigated its effect against arsenic-induced neurotoxicity and oxidative stress in mice. polyphenolics prepared from column chromatography of the crude methanol extract using diaion resin contained a phenolic content of 452.185 ± 7.879 mg gallic acid equivalent/gm of sample and flavonoid content of 200.075 ± 0.755 mg catechin equivalent/gm of sample. The polyphenolics exhibited potent antioxidant activity with respect to free radical scavenging ability, total antioxidant activity and inhibition of lipid peroxidation. Administration of arsenic in mice showed a reduction of acetylcholinesterase activity in the brain which was counteracted by polyphenolics. Similarly, elevation of lipid peroxidation and depletion of glutathione in the brain of mice was effectively restored to normal level by polyphenolics. Gallic acid methyl ester, catechin and catechin-7-gallate were identified in the polyphenolics as the major active compounds. These results suggest that polyphenolics due to its strong antioxidant potential might be effective in the management of arsenic induced neurotoxicity.