Development of an in vitro tissue culture system for hammer coral (Fimbriaphyllia ancora) ovaries

In vitro gonad culture systems have proven useful to investigate intrinsic mechanisms of sexual reproduction in animals. Here we describe development of an in vitro culture method for coral ovaries. Mesenterial tissues containing both ovaries and mesenterial filaments were microscopically isolated f...

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Bibliographic Details
Published in:Scientific reports 2021-12, Vol.11 (1), p.24338-24338, Article 24338
Main Authors: Chiu, Yi-Ling, Chang, Ching-Fong, Shikina, Shinya
Format: Article
Language:English
Subjects:
631/61
631/80
Ampicillin
Animals
Anthozoa
Antibiotics
Cell culture
Cell proliferation
Female
Filaments
Humanities and Social Sciences
In Vitro Techniques
multidisciplinary
Oocytes - cytology
Oocytes - metabolism
Oogenesis
Oogonia
Ovaries
Ovary - cytology
Ovary - metabolism
Penicillin
Science
Science (multidisciplinary)
Sexual reproduction
Somatic cells
Streptomycin
Tissue culture
Tissue Culture Techniques - methods
Vitellogenin
Vitellogenins - metabolism
Yolk protein
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Summary:In vitro gonad culture systems have proven useful to investigate intrinsic mechanisms of sexual reproduction in animals. Here we describe development of an in vitro culture method for coral ovaries. Mesenterial tissues containing both ovaries and mesenterial filaments were microscopically isolated from the scleractinian coral, Fimbriaphyllia ancora , and culture conditions were optimized. M199 diluted 10× (10% M199, pH 8.1) and supplemented with 25 mM HEPES and the antibiotics, ampicillin, penicillin and streptomycin, supported oocyte survival and maintained the structural integrity of ovaries during short-term culture (~ 6 days). Addition of a commercial antibiotic–antimycotic solution (Anti–Anti) and fetal bovine serum adversely affected ovary maintenance and caused tissue disintegration. Characterization of cultured ovaries showed that there is no difference in cell proliferation of ovarian somatic cells between culture Days 1 and 6. Moreover, the presence of oogonia and expression of a major yolk protein, vitellogenin, were confirmed in ovaries cultured for 6 days. This system will be useful for studying effects of a wide range of substances on coral oogenesis.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-03810-x