Comparative study of two Rift Valley fever virus field strains originating from Mauritania

• Published in: PLoS neglected tropical diseases 2024-12, Vol.18 (12), p.e0012728
• Main Authors: Chabert, Mehdi, Lacôte, Sandra, Marianneau, Philippe, Confort, Marie-Pierre, Aurine, Noémie, Pédarrieu, Aurélie, Doumbia, Baba, Ould Baba Ould Gueya, Mohamed, Habiboullah, Habiboullah, Beyatt, Ahmed Bezeid El Mamy, Lo, Modou Moustapha, Nichols, Jenna, Sreenu, Vattipally B, da Silva Filipe, Ana, Colle, Marie-Anne, Pain, Bertrand, Cêtre-Sossah, Catherine, Arnaud, Frédérick, Ratinier, Maxime
• Format: Article
• Language: English
• Subjects: Animals; Biology and life sciences; Camelus - virology; Computer and Information Sciences; Disease Outbreaks; Engineering and Technology; Genome, Viral; Goat Diseases - epidemiology; Goat Diseases - virology; Goats; Life Sciences; Mauritania - epidemiology; Medicine and health sciences; Mice; Mice, Inbred BALB C; Microbiology and Parasitology; Phylogeny; Physical Sciences; Research and Analysis Methods; Rift Valley Fever - epidemiology; Rift Valley Fever - transmission; Rift Valley Fever - virology; Rift Valley fever virus - classification; Rift Valley fever virus - genetics; Rift Valley fever virus - isolation & purification; Rift Valley fever virus - pathogenicity; Virology; Virulence
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0012728

Rift Valley fever (RVF) is one of the major viral arthropod-borne diseases in Africa. In recent decades, RVF virus (RVFV), the causative agent of RVF, has been responsible for multiple outbreaks in West Africa with important consequences on human and animal health. In particular, an outbreak occurred in 2010 after heavy rains in the desertic region of Adrar, Mauritania. It was characterized by the appearance of severe clinical signs among dromedary camels. Another one occurred in 2013-2014 across Senegal and the southern part of Mauritania. In this study, we characterized two RVFV field strains isolated during these two outbreaks. The first strain, MRU25010-30, was isolated from a camel (2010) while the second, MRU2687-3, was isolated from a goat (2013). By deep-sequencing and rapid amplification of cDNA-ends by polymerase chain reaction, we successfully sequenced the complete genome of these two RVFV strains as well as the reference laboratory strain ZH548. Phylogenetic analysis showed that the two field viruses belong to two different RVFV genetic lineages. Moreover, we showed that MRU25010-30 replicates more efficiently in various in vitro cell culture models than MRU2687-3 and ZH548. In vivo, MRU25010-30 caused rapid death of BALB/c mice and proved to be more virulent than MRU2687-3, regardless of the route of inoculation (subcutaneous or intranasal). The virulence of MRU25010-30 is associated with a high viral load in the liver and serum of infected mice, while the death of mice infected with MRU2687-3 and ZH548 correlated with a high viral load in the brain. Altogether, the data presented in this study provide new avenues to unveil the molecular viral determinants that modulate RVFV virulence and replication capacity.