Wolbachia wMel strain-mediated effects on dengue virus vertical transmission from Aedes aegypti to their offspring

Dengue virus serotypes (DENV-1 to -4) can be transmitted vertically in Aedes aegpti mosquitoes. Whether infection with the wMel strain of the endosymbiont Wolbachia can reduce the incidence of vertical transmission of DENV from infected females to their offspring is not well understood. A laboratory...

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Bibliographic Details
Published in:Parasites & vectors 2023-08, Vol.16 (1), p.308-6, Article 308
Main Authors: Duong Thi Hue, Kien, da Silva Goncalves, Daniela, Tran Thuy, Vi, Thi Vo, Long, Le Thi, Dui, Vu Tuyet, Nhu, Nguyen Thi, Giang, Huynh Thi Xuan, Trang, Nguyen Minh, Nguyet, Nguyen Thanh, Phong, Yacoub, Sophie, Simmons, Cameron P
Format: Article
Language:English
Subjects:
Adults
Aedes
Aedes aegypti
Agreements
Animals
Aquatic insects
Bacteria
Binomial distribution
Brief Report
Colonies
Culicidae
Dengue
Dengue fever
Dengue Virus
Disease transmission
Female
Females
Human diseases
Infections
Infectious Disease Transmission, Vertical
Laboratories
Maximum likelihood estimation
Microbiological strains
Mosquitoes
Offspring
Prevention
Risk factors
Serotypes
Statistical analysis
Sucrose
Vector-borne diseases
Vertical transmission
Viral infections
Viruses
wMel
Wolbachia
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Summary:Dengue virus serotypes (DENV-1 to -4) can be transmitted vertically in Aedes aegpti mosquitoes. Whether infection with the wMel strain of the endosymbiont Wolbachia can reduce the incidence of vertical transmission of DENV from infected females to their offspring is not well understood. A laboratory colony of Vietnamese Ae. aegypti, both with and without wMel infection, were infected with DENV-1 by intrathoracic injection (IT) to estimate the rate of vertical transmission (VT) of the virus. VT in the DENV-infected mosquitoes was calculated via the infection rate estimation from mosquito pool data using maximum likelihood estimation (MLE). In 6047 F1 Vietnamese wild-type Ae. aegypti, the MLE of DENV-1 infection was 1.49 per 1000 mosquitoes (95% confidence interval [CI] 0.73-2.74). In 5500 wMel-infected Ae. aegypti, the MLE infection rate was 0 (95% CI 0-0.69). The VT rates between mosquito lines showed a statistically significant difference. The results reinforce the view that VT is a rare event in wild-type mosquitoes and that infection with wMel is effective in reducing VT.
ISSN:1756-3305
1756-3305
DOI:10.1186/s13071-023-05921-y