Nkx2.5 marks angioblasts that contribute to hemogenic endothelium of the endocardium and dorsal aorta

Novel regenerative therapies may stem from deeper understanding of the mechanisms governing cardiovascular lineage diversification. Using enhancer mapping and live imaging in avian embryos, and genetic lineage tracing in mice, we investigated the spatio-temporal dynamics of cardiovascular progenitor...

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Bibliographic Details
Published in:eLife 2017-03, Vol.6
Main Authors: Zamir, Lyad, Singh, Reena, Nathan, Elisha, Patrick, Ralph, Yifa, Oren, Yahalom-Ronen, Yfat, Arraf, Alaa A, Schultheiss, Thomas M, Suo, Shengbao, Han, Jing-Dong Jackie, Peng, Guangdun, Jing, Naihe, Wang, Yuliang, Palpant, Nathan, Tam, Patrick Pl, Harvey, Richard P, Tzahor, Eldad
Format: Article
Language:English
Subjects:
Animals
Aorta
Aorta - embryology
Biology
Blood
cardiogenesis
CD45 antigen
Cell lineage
Chick Embryo
Coronary vessels
Developmental Biology and Stem Cells
Ectopic expression
Embryos
Endocardium - embryology
Endothelium
Gene expression
Gene mapping
Heart
Heart diseases
Hemangioblasts - physiology
hemogenic endothelium
Homeobox Protein Nkx-2.5 - metabolism
Laboratories
Liver
Mesoderm
Mice
Nkx2.5
Nkx2.5 protein
Rodents
Runx1 protein
Spatio-Temporal Analysis
Stem cells
Transcription factors
Zebrafish
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Summary:Novel regenerative therapies may stem from deeper understanding of the mechanisms governing cardiovascular lineage diversification. Using enhancer mapping and live imaging in avian embryos, and genetic lineage tracing in mice, we investigated the spatio-temporal dynamics of cardiovascular progenitor populations. We show that expression of the cardiac transcription factor marks a mesodermal population outside of the cardiac crescent in the extraembryonic and lateral plate mesoderm, with characteristics of hemogenic angioblasts. Extra-cardiac lineage progenitors migrate into the embryo and contribute to clusters of CD41 /CD45 and RUNX1 cells in the endocardium, the aorta-gonad-mesonephros region of the dorsal aorta and liver. We also demonstrated that ectopic expression of in chick embryos activates the hemoangiogenic gene expression program. Taken together, we identified a hemogenic angioblast cell lineage characterized by transient expression that contributes to hemogenic endothelium and endocardium, suggesting a novel role for in hemoangiogenic lineage specification and diversification.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.20994