Volatile constituents of Amomum argyrophyllum Ridl. and Amomum dealbatum Roxb. and their antioxidant, tyrosinase inhibitory and cytotoxic activities

• Published in: Arabian journal of chemistry 2022-10, Vol.15 (10), p.104148, Article 104148
• Main Authors: Pintatum, Aknarin, Laphookhieo, Surat
• Format: Article
• Language: English
• Subjects: Amomum argryllophitum; Amomum delbatum; Anti-inflammatory; Antioxidant activity; Chemical composition; Tyrosinase inhibitory activity
• ISSN: 1878-5352; 1878-5379
• DOI: 10.1016/j.arabjc.2022.104148

The volatile components from fresh rhizomes and leaves of Amomum argyrophyllum Ridl. and Amomum dealbatum Roxb. were performed using HS-SPME and charac-terized by GC–MS. A total of 49, 47, 49, and 34 compounds were identified from the rhizomes and leaves of A. argyrophyllum and A. dealbatum, respectively. The major components were β-pinene, α-pinene, and o-cymene. The rhizome extracts exhibited total phenolic content of 2.9 ± 0.5 and 2.1 ± 0.6 mg gallic acid equivalents. The IC50 values of DPPH and ABTS were 179.8 ± 3.9 µg/mL, 392.9 ± 2.6 µg/mL, 120.3 ± 2.5 µg/mL, and 328.6 ± 3.3 µg/mL, respectively. The FRAP values were 76.5 ± 7.8 and 84.9 ± 4.4 µM ascorbic acid equivalents. The extracts showed weak antibacterial activity and tyrosinase inhibitory activity of 69.0 ± 3.6 and 53.7 ± 7.4 mg kojic acid equivalents. The cytotoxicity effect was assessed with the MTT assay at 200 µg/mL. The extracts showed no toxicity. In addition, the anti-inflammatory properties of extracts were evaluated, and showed potential to inhibit nuclear factor-κB (NF-κB) activity.