Modulation of human endogenous retroviruses and cytokines expression in peripheral blood mononuclear cells from autistic children and their parents

• Published in: Retrovirology 2022-11, Vol.19 (1), p.26-26, Article 26
• Main Authors: Cipriani, Chiara, Giudice, Martina, Petrone, Vita, Fanelli, Marialaura, Minutolo, Antonella, Miele, Martino T, Toschi, Nicola, Maracchioni, Christian, Siracusano, Martina, Benvenuto, Arianna, Coniglio, Antonella, Curatolo, Paolo, Mazzone, Luigi, Sandro, Grelli, Garaci, Enrico, Sinibaldi-Vallebona, Paola, Matteucci, Claudia, Balestrieri, Emanuela
• Format: Article
• Language: English
• Subjects: Antiretroviral agents; Antiretrovirals; Autism; Autism Spectrum Disorder; Autistic children; Autistic Disorder; Child; Children; Cytokines; Divalproex; Efavirenz; Endogenous Retroviruses - genetics; Gene expression; Genes; Genetic engineering; Genotype & phenotype; HEMO; HERVs; Highly active antiretroviral therapy; Humans; Immune system; Immunosuppressive agents; In vitro treatment; Inflammation; Interleukin 2; Interleukins; Leukocytes, Mononuclear; Mental disorders; Mental illness; Microbiota; Mothers; Mutation; Nervous system; Neurological diseases; Parents; Peripheral blood mononuclear cells; Phytohemagglutinins; Retroviruses; Tumor necrosis factor-TNF; Valproic acid; Valproic Acid - pharmacology
• ISSN: 1742-4690; 1742-4690
• DOI: 10.1186/s12977-022-00603-6

Putative pathogenic effects mediated by human endogenous retroviruses (HERVs) in neurological and psychiatric disorders in humans have been extensively described. HERVs may alter the development of the brain by means of several mechanisms, including modulation of gene expression, alteration of DNA stability, and activation of immune system. We recently demonstrated that autistic children and their mothers share high expression levels of some HERVs and cytokines in peripheral blood mononuclear cells (PBMCs) ex vivo, suggesting a close mother-child association in Autism Spectrum Disorder (ASD). In the present study, PBMCs from autistic children and their parents were exposed to stimulating factors (Interleukin-2/Phytohaemagglutinin) or drugs, as Valproic acid and Efavirenz. The results show that HERVs and cytokines expression can be modulated in vitro by different stimuli in PBMCs from autistic children and their mothers, while no significant changes were found in PBMCs ASD fathers or in controls individuals. In particular, in vitro exposure to interleukin-2/Phytohaemagglutinin or valproic acid induces the expression of several HERVs and cytokines while Efavirenz inhibits them. Herein we show that autistic children and their mothers share an intrinsic responsiveness to in vitro microenvironmental changes in expressing HERVs and pro-inflammatory cytokines. Remarkably, the antiretroviral drug Efavirenz restores the expression of specific HERV families to values similar to those of the controls, also reducing the expression of proinflammatory cytokines but keeping the regulatory ones high. Our findings open new perspectives to study the role of HERVs in the biological mechanisms underlying Autism.