Olive Leaf Mottling Virus: A New Member of the Genus Olivavirus

• Published in: Plants (Basel) 2024-08, Vol.13 (16), p.2290
• Main Authors: Ruiz-García, Ana Belén, Candresse, Thierry, Malagón, José, Ruiz-Torres, Manuel, Paz, Sergio, Pérez-Sierra, Ana, Olmos, Antonio
• Format: Article
• Language: English
• Subjects: Amino acids; Closteroviridae; common names; Crop diseases; family; Fruit trees; Fruits; Gene sequencing; genome; Genomes; genus; HTS; Leaves; Life Sciences; New species; Next-generation sequencing; nucleotide sequences; Olivavirus; olive; olives; OLMV; Open reading frames; Phylogeny; Phytopathology and phytopharmacy; Proteins; RNA; RNA polymerase; RNA viruses; species; Vegetal Biology; Vernacular names; virome; Viruses; Yellowing
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13162290

Studies of the virome of olive trees with symptoms of leaf mottling by high-throughput sequencing (HTS) revealed the presence of a new virus. Full coding genome sequences of two isolates were determined and consisted of a single RNA segment of 16,516 nt and 16,489, respectively. The genomic organization contained 10 open reading frames (ORFs) from 5' to 3': ORF1a, ORF1b (RdRp), ORF2 (p22), ORF3 (p7), ORF4 (HSP70h), ORF5 (HSP90h), ORF6 (CP), ORF7 (p19), ORF8 (p12), ORF9 (p23) and ORF10 (p9). Phylogenetic analyses clustered this virus in the genus , family with the closest species being , commonly named olive leaf yellowing-associated virus (OLYaV). However, amino acid sequences of all taxonomically relevant proteins showed, in all cases, a divergence higher than 25% between OLYaV and the new virus, indicating that it represents a new species in the genus for which the common name of olive leaf mottling virus (OLMV) is proposed. This study represents an advance in the genus and provides new insights into the olive virome.