Circulating tumor cells detected with a microcavity array predict clinical outcome in hepatocellular carcinoma

The present study aimed to establish a novel isolation strategy for circulating tumor cells (CTCs) using a microcavity array (MCA) system and to evaluate the clinical significance of CTCs in hepatocellular carcinoma (HCC). We examined recovery rates of HCC cell lines spiked into whole blood in MCA a...

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Bibliographic Details
Published in:Cancer medicine (Malden, MA) MA), 2021-04, Vol.10 (7), p.2300-2309
Main Authors: Takahashi, Kazuto, Ofuji, Kazuya, Hiramatsu, Katsushi, Nosaka, Takuto, Naito, Tatsushi, Matsuda, Hidetaka, Endo, Katsuya, Higuchi, Masayuki, Ohtani, Masahiro, Nemoto, Tomoyuki, Nakamoto, Yasunari
Format: Article
Language:English
Subjects:
albumin
Antibodies
Blood
Cell adhesion & migration
Cirrhosis
Clinical Cancer Research
Clinical outcomes
CTC
Cytokeratin
EpCAM
Experiments
FDA approval
Gene expression
HCC
Heparan sulfate proteoglycans
Hepatitis
Hepatocellular carcinoma
Liver cancer
Liver cirrhosis
Medical prognosis
Metastases
Metastasis
microcavity array
Mortality
Original Research
Peripheral blood
Tumor cells
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Summary:The present study aimed to establish a novel isolation strategy for circulating tumor cells (CTCs) using a microcavity array (MCA) system and to evaluate the clinical significance of CTCs in hepatocellular carcinoma (HCC). We examined recovery rates of HCC cell lines spiked into whole blood in MCA assay. Circulating tumor cells were isolated from peripheral blood samples (3 mL) of 7 healthy donors (HD), 14 patients with liver cirrhosis (LC), and 31 patients with HCC using the MCA system. Additionally, we investigated the mRNA expression of liver‐specific genes in isolated CTCs using qPCR. The recovery rates were 65.1% (HepG2), 76.7% (HuH7), and 99.0% (PLC/PRF/5). In HD and patients with LC and HCC, the CTC positivity rate (CTCs ≥10) and average CTC number were as follows: HD 0% and 0.1, LC 14.3% and 5.3, HCC 54.8% and 47.6, respectively. The CTC positivity rate in HCC was significantly higher than that in LC (p 
ISSN:2045-7634
2045-7634
DOI:10.1002/cam4.3790