Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis

South Africa remains challenged with a high tuberculosis burden accompanied by an increase in drug resistant cases. We assessed the use of the Illumina MiSeq, a next-generation sequencing platform for whole genome sequencing, followed by bioinformatic analysis using a commercial software package to...

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Bibliographic Details
Published in:African journal of laboratory medicine 2019, Vol.8 (1), p.1-5
Main Authors: Ismail, Nazir A., Said, Halima M., Ismail, Farzana, Ismail, Nabila, Omar, Shaheed V., Gwala, Thabisile L., Joseph, Lavania
Format: Article
Language:English
Subjects:
Brief Report
Drug resistance
Genomes
Genotype & phenotype
Laboratories
Medical Laboratory Technology
Mutation
Tuberculosis
whole genome sequence
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Summary:South Africa remains challenged with a high tuberculosis burden accompanied by an increase in drug resistant cases. We assessed the use of the Illumina MiSeq, a next-generation sequencing platform for whole genome sequencing, followed by bioinformatic analysis using a commercial software package to determine resistance to selected drugs used for Mycobacterium tuberculosis treatment in our setting. Whole genome sequencing shows potential as a diagnostic platform for the detection of drug resistance in Mycobacterium tuberculosis with the provision of information for several drugs simultaneously.
ISSN:2225-2002
2225-2010
2225-2010
DOI:10.4102/ajlm.v8i1.801