Optimization of the Use of Industrial Wastes in Anaerobic Soil Disinfestation for the Control of Fusarium Wilt in Strawberry

• Published in: Plants (Basel) 2023-09, Vol.12 (18), p.3185
• Main Authors: Hernández-Muñiz, Paloma, Borrero, Celia, Ordóñez-Martín, Javier, Pastrana, Ana M, Avilés, Manuel
• Format: Article
• Language: English
• Subjects: Agricultural research; Agricultural wastes; Anaerobic conditions; Anaerobiosis; Carbon; Control; Disease; disease severity; Diseases and pests; Fish meal; fishmeal; Fruits; Fumigants; Fusarium; Fusarium oxysporum f. sp. fragariae; Growth chambers; Industrial wastes; Methods; Microorganisms; Optimization; Pathogens; Physiological aspects; Population density; Reduction; residual strawberry extrudate; Rice; Rice bran; Signs and symptoms; Soil disinfection; Soil treatment; Soils; Strawberries; Wilt
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12183185

Anaerobic soil disinfestation (ASD) is proposed as an alternative to the use of chemical fumigants against Fusarium wilt in strawberry crops. Different residual wastes (rice bran, fishmeal, and residual strawberry extrudate) were assessed as amendments for ASD. Two different concentrations and two incubation durations were tested in growth chamber trials. The abundance of several microbial groups was noted before and after the treatments. Strawberry plants were grown in the treated soils to record Fusarium wilt disease severity. The population density of F. oxysporum increased after ASD in most amendments with rice bran and residual strawberry extrudate. Changes in Trichoderma spp., copiotrophic bacteria, and Streptomyces spp. populations were observed after anaerobiosis treatments and plant trials. A reduction in the disease severity was achieved in ASD-treated soils with 20 t/ha of rice bran at both 25 and 60 days of incubation, but not when using a 13.5 t/ha dose. Similarly, treatments using 19.3 t/ha of fishmeal for both incubation durations were able to reduce disease severity. In contrast, a severity reduction was only obtained in soils treated with 25.02 t/ha of the residual strawberry extrudate and incubated for 60 days in anaerobic conditions. Two of the three by-products tested were able to reduce Fusarium wilt symptoms in strawberry plants after an ASD-treatment period of only 25 days. Accordingly, the technique seems promising for strawberry growers in Huelva, Spain, and highly sustainable by giving value to residues produced in surrounding areas.