Simple formulation and characterization of double emulsion variant designed to carry three bioactive agents

Multiple emulsions are thermodynamically stable systems that mark applications in various fields including drug delivery systems. They allow enhanced availability of drugs, greater absorption, and present reduced toxicity, among other desirable properties. In this work, we aimed to formulate a uniqu...

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Bibliographic Details
Published in:Heliyon 2022-09, Vol.8 (9), p.e10397-e10397, Article e10397
Main Authors: Hema, S.K., Karmakar, Aparajita, Das, Raunak Kumar, Srivastava, Priyanka
Format: Article
Language:English
Subjects:
Bright field microscopy
Disc diffusion
DLS
FT-IR
Fusobacterium nucleatum
HR-TEM
Image processing
Multiple emulsions' double emulsion variant
Porphyromonas gingivalis
Synthesis
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Summary:Multiple emulsions are thermodynamically stable systems that mark applications in various fields including drug delivery systems. They allow enhanced availability of drugs, greater absorption, and present reduced toxicity, among other desirable properties. In this work, we aimed to formulate a unique double emulsion (O1/W + W1/O2/W/W) with three bioactive components viz. Ocimum tenuiflorum oil, Cocos nucifera oil and crystalline Cinnamomum camphora. Three surfactants with different HLB values viz. Tween-20, Tween-80 and Triton X-100 were used for the emulsification process. The method followed was simple as compared to current methods employed for formulating multiple emulsions. Formulation was characterized using techniques of bright field microscopy, Dynamic Light Scattering (DLS), High-Resolution Transmission Electron Microscopy (HR-TEM) and Fourier-transform infrared spectroscopy (FTIR). Image processing tools were also used to characterize the formulation, which reliably cross-verified the observations from conventional characterization techniques. The potency of individual components of emulsion was compared with the prepared double emulsion model by testing the activity on two pathologically relevant bacterial strains: Fusobacterium nucleatum (FN) and Porphyromonas gingivalis (PG). Multiple emulsions' double emulsion variant, Synthesis, Bright field microscopy, DLS, FT-IR, HR-TEM, Image processing, Disc diffusion, Fusobacterium nucleatum, Porphyromonas gingivalis
ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2022.e10397