Drought-induced assembly of rhizosphere mycobiomes shows beneficial effects on plant growth

• Published in: mSystems 2024-07, Vol.9 (7), p.e0035424
• Main Authors: Pan, Yanshuo, Liu, Binhui, Zhang, Wenying, Zhuang, Shan, Wang, Hongzhe, Chen, Jieyin, Xiao, Liang, Li, Yuzhong, Han, Dongfei
• Format: Article
• Language: English
• Subjects: Abscisic acid; Adaptability; Agricultural production; Crop production; Drought; Droughts; Endangered & extinct species; Environmental Microbiology; Experiments; fungal community assembly; Fungi - physiology; generalists; Host plants; Irrigation; Microbiomes; Microorganisms; Mycobiome; Phylogenetics; Plant Development - physiology; Plant growth; Plant Roots - microbiology; Research Article; Rhizosphere; Soil Microbiology; specialists; Species richness; Stochastic models; Stochasticity; Stress, Physiological; Taxonomy; Trees; Triticum - growth & development; Triticum - microbiology; Variance analysis; Wheat
• ISSN: 2379-5077; 2379-5077
• DOI: 10.1128/msystems.00354-24

Beneficial interactions between plants and rhizosphere fungi can enhance plant adaptability during drought stress. However, harnessing these interactions will require an in-depth understanding of the response of fungal community assembly to drought. Herein, by using different varieties of wheat plants, we analyzed the drought-induced changes in fungal community assembly in rhizosphere and bulk soil. We demonstrated that drought significantly altered the fungal communities, with the contribution of species richness to community beta diversity increased in both rhizosphere and bulk soil compartments during drought stress. The stochastic processes dominated fungal community assembly, but the relative importance of deterministic processes, mainly homogeneous selection, increased in the drought-stressed rhizosphere. Drought induced an increase in the relative abundance of generalists in the rhizosphere, as opposed to specialists, and the top 10 abundant taxa that enriched under drought conditions were predominantly generalists. Notably, the most abundant drought-enriched taxon in rhizosphere was a generalist, and the corresponding strain was found capable of improving root length and activating ABA signaling in wheat plants through culture-based experiment. Together, these findings provide evidence that host plants exert a strong influence on rhizospheric fungal community assembly during stress and suggest the fungal communities that have experienced drought have the potential to confer fitness advantages to the host plants. We have presented a framework to integrate the shifts in community assembly processes with plant-soil feedback during drought stress. We found that environmental filtering and host plant selection exert influence on the rhizospheric fungal community assembly, and the re-assembled community has great potential to alleviate plant drought stress. Our study proposes that future research should incorporate ecology with plant, microbiome, and molecular approaches to effectively harness the rhizospheric microbiome for enhancing the resilience of crop production to drought.