Investigation of SAMD1 ablation in mice

SAM domain-containing protein 1 (SAMD1) has been implicated in atherosclerosis, as well as in chromatin and transcriptional regulation, suggesting a versatile and complex biological function. However, its role at an organismal level is currently unknown. Here, we generated SAMD1 −/− and SAMD1 +/− mi...

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Published in:Scientific reports 2023-02, Vol.13 (1), p.3000-3000, Article 3000
Main Authors: Campbell, Bruce, Weber, Lisa M., Engle, Sandra J., Ozolinš, Terence R. S., Bourassa, Patricia, Aiello, Robert, Liefke, Robert
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631/337/100
Animals
Arteriosclerosis
Blood vessels
Chromatin
Embryo, Mammalian - metabolism
Embryogenesis
Embryonic Development
Embryonic growth stage
Embryos
Erythrocytes
Gene regulation
Genotyping
Heterozygote
Homozygote
Humanities and Social Sciences
Mice
Mice, Knockout
multidisciplinary
Science
Science (multidisciplinary)
Steroidogenesis
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creator Campbell, Bruce
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Liefke, Robert
description SAM domain-containing protein 1 (SAMD1) has been implicated in atherosclerosis, as well as in chromatin and transcriptional regulation, suggesting a versatile and complex biological function. However, its role at an organismal level is currently unknown. Here, we generated SAMD1 −/− and SAMD1 +/− mice to explore the role of SAMD1 during mouse embryogenesis. Homozygous loss of SAMD1 was embryonic lethal, with no living animals seen after embryonic day 18.5. At embryonic day 14.5, organs were degrading and/or incompletely developed, and no functional blood vessels were observed, suggesting failed blood vessel maturation. Sparse red blood cells were scattered and pooled, primarily near the embryo surface. Some embryos had malformed heads and brains at embryonic day 15.5. In vitro, SAMD1 absence impaired neuronal differentiation processes. Heterozygous SAMD1 knockout mice underwent normal embryogenesis and were born alive. Postnatal genotyping showed a reduced ability of these mice to thrive, possibly due to altered steroidogenesis. In summary, the characterization of SAMD1 knockout mice suggests a critical role of SAMD1 during developmental processes in multiple organs and tissues.
doi_str_mv 10.1038/s41598-023-29779-3
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subjects 631/136
631/337/100
Animals
Arteriosclerosis
Blood vessels
Chromatin
Embryo, Mammalian - metabolism
Embryogenesis
Embryonic Development
Embryonic growth stage
Embryos
Erythrocytes
Gene regulation
Genotyping
Heterozygote
Homozygote
Humanities and Social Sciences
Mice
Mice, Knockout
multidisciplinary
Science
Science (multidisciplinary)
Steroidogenesis
title Investigation of SAMD1 ablation in mice
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