Isolation of Alkaloids from Sinomenium acutum by Centrifugal Partition Chromatography and Their Ameliorating Effects on Dexamethasone-Induced Atrophy in C2C12 Myotubes

Bioactivity-guided isolation was conducted using centrifugal partition chromatography (CPC) from an extract of Sinomenium acutum rhizome, which has shown promising preventive effects in a dexamethasone-induced C2C12 myotube atrophy model. CPC was operated with a solvent system of n-butanol–acetonitr...

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Bibliographic Details
Published in:Separations 2023-09, Vol.10 (9), p.470
Main Authors: Jung, Eun Ju, Kim, Ji Hoon, Kim, Hye Mi, Guo, Shuo, Lee, Do Hyun, Lim, Gyu Min, Syed, Ahmed Shah, Kim, Wondong, Kim, Chul Young
Format: Article
Language:English
Subjects:
Acetonitrile
Acids
acutumine
Alkaloids
Atrophy
Butanol
centrifugal partition chromatography
Chromatography
Dexamethasone
Glycosides
High performance liquid chromatography
Ions
Lignans
magnoflorine
Musculoskeletal system
Penicillin
sinomenine
Sinomenium acutum
skeletal muscle atrophy
Solvents
Triethylamine
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Summary:Bioactivity-guided isolation was conducted using centrifugal partition chromatography (CPC) from an extract of Sinomenium acutum rhizome, which has shown promising preventive effects in a dexamethasone-induced C2C12 myotube atrophy model. CPC was operated with a solvent system of n-butanol–acetonitrile–water (10:2:8, v/v/v, containing 0.5% triethylamine) in dual mode (ascending to descending), which provided a high recovery rate (>99%) with a high resolution. Then, the preventive effects of the obtained CPC fractions were examined against dexamethasone-induced atrophy in C2C12 myotubes according to the weight ratios of the obtained fractions. The active fractions were further purified by semi-preparative HPLC that led to obtaining five alkaloids, one lignan glycoside, and one phenylpropanoid glycoside. Among these, at a concentration of 1 nM, sinomenine, magnoflorine, and acutumine could ameliorate dexamethasone-induced myotube atrophy in C2C12 myotubes by 9.3%, 13.8%, and 11.3%, respectively.
ISSN:2297-8739
2297-8739
DOI:10.3390/separations10090470