Morphological and phylogenetic analyzes reveal two new species of Melanconiella from Fujian Province, China

• Published in: Frontiers in microbiology 2023-08, Vol.14, p.1229705-1229705
• Main Authors: Mu, Taichang, Chen, Jinhui, Zhao, Zhiying, Zhang, Weibin, Stephenson, Steven L., Yang, Chenjie, Zhu, Mengjia, Su, Hailan, Liu, Pu, Guan, Xiayu, Qiu, Junzhi
• Format: Article
• Language: English
• Subjects: leaf disease; Melanconiellaceae; Microbiology; multi-gene phylogeny; new species; taxonomy
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2023.1229705

IntroductionSpecies of Melanconiella include a diverse array of plant pathogens as well as endophytic fungi. Members of this genus have been frequently collected from the family Betulaceae (birches) in Europe and North America. Little, however, if known concerning the distribution of Melanconiella and/or their potential as pathogens of other plant hosts. MethodsFungi were noted and isolated from diseased leaves of Loropetalum chinense (Chinese fringe flower) and Camellia sinensis (tea) in Fujian Province, China. Genomic DNA was extracted from fungal isolates and the nucleotide sequences of four loci were determined and sued to construct phylogenetic trees. Morphological characteristics of fungal structures were determined via microscopic analyses. ResultsFour strains and two new species of Melanconiella were isolated from infected leaves of L. chinense and C. sinensis in Fujian Province, China. Based on morphology and a multi-gene phylogeny of the internal transcribed spacer regions with the intervening 5.8S nrRNA gene (ITS), the 28S large subunit of nuclear ribosomal RNA (LSU), the second largest subunit of RNA polymerase II (RPB2), and the translation elongation factor 1-α gene (TEF1-α), Melanconiellaloropetali sp. nov. and Melanconiellacamelliae sp. nov. were identified and described herein. Detailed descriptions, illustrations, and a key to the known species of Melanconiella are provided. DiscussionThese data identify new species of Melanconiella, expanding the potential range and distribution of these dark septate fungi. The developed keys provide a reference source for further characterization of these fungi.