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Retrospective analysis of Vibrio spp. isolated from marketed crustaceans using multilocus sequence analysis
The genus includes bacteria with different morphological and metabolic characteristics responsible for different human and animal diseases. An accurate identification is essential to assess the risks in regard to aquatic organisms and consequently to public health. The Multilocus Sequence Analysis (...
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| Published in: | Italian journal of food safety 2023-03, Vol.12 (1), p.11045-11045 |
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| Main Authors: | , , , , , , , , , |
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| Language: | English |
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| container_title | Italian journal of food safety |
| container_volume | 12 |
| creator | Rahman, Mohammad Shamsur Currò, Sarah Carraro, Lisa Cardazzo, Barbara Balzan, Stefania Novelli, Enrico Fontana, Federico Caburlotto, Greta Manfrin, Amedeo Fasolato, Luca |
| description | The genus
includes bacteria with different morphological and metabolic characteristics responsible for different human and animal diseases. An accurate identification is essential to assess the risks in regard to aquatic organisms and consequently to public health. The Multilocus Sequence Analysis (MLSA) scheme developed on the basis of 4 housekeeping genes (
B,
H,
A and
A) was applied to identify 92
strains isolated from crustaceans in 2011. Concatenated sequences were used for the phylogenetic and population analyses and the results were compared with those from biochemical identification tests. From the phylogenetic analysis, 10 clusters and 4 singletons emerged, whereas the population analysis highlighted 12 subpopulations that were well supported by phylogeny with few exceptions. The retrospective analysis allowed correct re-attribution of isolated species, indicating how, for some pathogens, there may be an overestimation of phenotypic identification (
.
). Use of the PubMLST
database highlighted a possible genetic link between Sequence Type (ST) 529 and ST195 (
) isolated from a human case in Norway during 2018. In addition to the identification of major risk groups of
,
and
, MLSA could be a valid support for species considered a minor risk, such as
,
and
. Due to the increased incidence of vibriosis in Europe, the application of different tools will also have to be considered to investigate the possible epidemiological links of the various species in the perspective of
to protect the consumer. |
| doi_str_mv | 10.4081/ijfs.2023.11045 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_930681a033084d27a938ffae4d1e0d21</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_930681a033084d27a938ffae4d1e0d21</doaj_id><sourcerecordid>2802427036</sourcerecordid><originalsourceid>FETCH-LOGICAL-c419t-6962e130a834e52373420825091db503ab39bae26bb5a5f7b5e2f3e4bf76de803</originalsourceid><addsrcrecordid>eNpVkUtv1DAURiMEolXpmh3yks1Mr6-dh1cIVTwqVUJCwNa6ca4HTzNxsJNK_fcknVLalV-fjz_5FMVbCVsNjbwIe5-3CKi2UoIuXxSniMpsaqnw5ZP5SXGe8x4AJBqDJb4uTlQNlS4RT4ub7zylmEd2U7hlQQP1dzlkEb34FdoUosjjuBUhx54m7oRP8SAOlG54Xbk054kc05DFnMOwE4e5n0If3ZxF5j8zD-4_9E3xylOf-fxhPCt-fv704_Lr5vrbl6vLj9cbp6WZNpWpkKUCapTmElWtNEKDJRjZtSUoapVpibFq25JKX7clo1esW19XHTegzoqrI7eLtLdjCkvfOxsp2PuNmHaW0hRcz9YoqBpJoBQ0usOajGq8J9adZOhQLqwPR9Y4twfuHA9Tov4Z9PnJEH7bXby1EiSgqdY27x8IKS4fkid7CNlx39PAcc4WG0CNNahqiV4co25RkhP7x3ck2FW5XZXbVbm9V77cePe03mP-n2D1F7Fmqas</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2802427036</pqid></control><display><type>article</type><title>Retrospective analysis of Vibrio spp. isolated from marketed crustaceans using multilocus sequence analysis</title><source>PubMed Central</source><creator>Rahman, Mohammad Shamsur ; Currò, Sarah ; Carraro, Lisa ; Cardazzo, Barbara ; Balzan, Stefania ; Novelli, Enrico ; Fontana, Federico ; Caburlotto, Greta ; Manfrin, Amedeo ; Fasolato, Luca</creator><creatorcontrib>Rahman, Mohammad Shamsur ; Currò, Sarah ; Carraro, Lisa ; Cardazzo, Barbara ; Balzan, Stefania ; Novelli, Enrico ; Fontana, Federico ; Caburlotto, Greta ; Manfrin, Amedeo ; Fasolato, Luca</creatorcontrib><description>The genus
includes bacteria with different morphological and metabolic characteristics responsible for different human and animal diseases. An accurate identification is essential to assess the risks in regard to aquatic organisms and consequently to public health. The Multilocus Sequence Analysis (MLSA) scheme developed on the basis of 4 housekeeping genes (
B,
H,
A and
A) was applied to identify 92
strains isolated from crustaceans in 2011. Concatenated sequences were used for the phylogenetic and population analyses and the results were compared with those from biochemical identification tests. From the phylogenetic analysis, 10 clusters and 4 singletons emerged, whereas the population analysis highlighted 12 subpopulations that were well supported by phylogeny with few exceptions. The retrospective analysis allowed correct re-attribution of isolated species, indicating how, for some pathogens, there may be an overestimation of phenotypic identification (
.
). Use of the PubMLST
database highlighted a possible genetic link between Sequence Type (ST) 529 and ST195 (
) isolated from a human case in Norway during 2018. In addition to the identification of major risk groups of
,
and
, MLSA could be a valid support for species considered a minor risk, such as
,
and
. Due to the increased incidence of vibriosis in Europe, the application of different tools will also have to be considered to investigate the possible epidemiological links of the various species in the perspective of
to protect the consumer.</description><identifier>ISSN: 2239-7132</identifier><identifier>EISSN: 2239-7132</identifier><identifier>DOI: 10.4081/ijfs.2023.11045</identifier><identifier>PMID: 37064522</identifier><language>eng</language><publisher>Italy: PAGEPress Publications, Pavia, Italy</publisher><subject>crustacean ; emerging risks ; MLSA ; Vibrio spp</subject><ispartof>Italian journal of food safety, 2023-03, Vol.12 (1), p.11045-11045</ispartof><rights>Copyright: the Author(s).</rights><rights>Copyright: the Author(s) 2023 Licensee PAGEPress, Italy</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-6962e130a834e52373420825091db503ab39bae26bb5a5f7b5e2f3e4bf76de803</citedby><cites>FETCH-LOGICAL-c419t-6962e130a834e52373420825091db503ab39bae26bb5a5f7b5e2f3e4bf76de803</cites><orcidid>0000-0002-7746-1150</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102960/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102960/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37064522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rahman, Mohammad Shamsur</creatorcontrib><creatorcontrib>Currò, Sarah</creatorcontrib><creatorcontrib>Carraro, Lisa</creatorcontrib><creatorcontrib>Cardazzo, Barbara</creatorcontrib><creatorcontrib>Balzan, Stefania</creatorcontrib><creatorcontrib>Novelli, Enrico</creatorcontrib><creatorcontrib>Fontana, Federico</creatorcontrib><creatorcontrib>Caburlotto, Greta</creatorcontrib><creatorcontrib>Manfrin, Amedeo</creatorcontrib><creatorcontrib>Fasolato, Luca</creatorcontrib><title>Retrospective analysis of Vibrio spp. isolated from marketed crustaceans using multilocus sequence analysis</title><title>Italian journal of food safety</title><addtitle>Ital J Food Saf</addtitle><description>The genus
includes bacteria with different morphological and metabolic characteristics responsible for different human and animal diseases. An accurate identification is essential to assess the risks in regard to aquatic organisms and consequently to public health. The Multilocus Sequence Analysis (MLSA) scheme developed on the basis of 4 housekeeping genes (
B,
H,
A and
A) was applied to identify 92
strains isolated from crustaceans in 2011. Concatenated sequences were used for the phylogenetic and population analyses and the results were compared with those from biochemical identification tests. From the phylogenetic analysis, 10 clusters and 4 singletons emerged, whereas the population analysis highlighted 12 subpopulations that were well supported by phylogeny with few exceptions. The retrospective analysis allowed correct re-attribution of isolated species, indicating how, for some pathogens, there may be an overestimation of phenotypic identification (
.
). Use of the PubMLST
database highlighted a possible genetic link between Sequence Type (ST) 529 and ST195 (
) isolated from a human case in Norway during 2018. In addition to the identification of major risk groups of
,
and
, MLSA could be a valid support for species considered a minor risk, such as
,
and
. Due to the increased incidence of vibriosis in Europe, the application of different tools will also have to be considered to investigate the possible epidemiological links of the various species in the perspective of
to protect the consumer.</description><subject>crustacean</subject><subject>emerging risks</subject><subject>MLSA</subject><subject>Vibrio spp</subject><issn>2239-7132</issn><issn>2239-7132</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVkUtv1DAURiMEolXpmh3yks1Mr6-dh1cIVTwqVUJCwNa6ca4HTzNxsJNK_fcknVLalV-fjz_5FMVbCVsNjbwIe5-3CKi2UoIuXxSniMpsaqnw5ZP5SXGe8x4AJBqDJb4uTlQNlS4RT4ub7zylmEd2U7hlQQP1dzlkEb34FdoUosjjuBUhx54m7oRP8SAOlG54Xbk054kc05DFnMOwE4e5n0If3ZxF5j8zD-4_9E3xylOf-fxhPCt-fv704_Lr5vrbl6vLj9cbp6WZNpWpkKUCapTmElWtNEKDJRjZtSUoapVpibFq25JKX7clo1esW19XHTegzoqrI7eLtLdjCkvfOxsp2PuNmHaW0hRcz9YoqBpJoBQ0usOajGq8J9adZOhQLqwPR9Y4twfuHA9Tov4Z9PnJEH7bXby1EiSgqdY27x8IKS4fkid7CNlx39PAcc4WG0CNNahqiV4co25RkhP7x3ck2FW5XZXbVbm9V77cePe03mP-n2D1F7Fmqas</recordid><startdate>20230308</startdate><enddate>20230308</enddate><creator>Rahman, Mohammad Shamsur</creator><creator>Currò, Sarah</creator><creator>Carraro, Lisa</creator><creator>Cardazzo, Barbara</creator><creator>Balzan, Stefania</creator><creator>Novelli, Enrico</creator><creator>Fontana, Federico</creator><creator>Caburlotto, Greta</creator><creator>Manfrin, Amedeo</creator><creator>Fasolato, Luca</creator><general>PAGEPress Publications, Pavia, Italy</general><general>PAGEPress Publications</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-7746-1150</orcidid></search><sort><creationdate>20230308</creationdate><title>Retrospective analysis of Vibrio spp. isolated from marketed crustaceans using multilocus sequence analysis</title><author>Rahman, Mohammad Shamsur ; Currò, Sarah ; Carraro, Lisa ; Cardazzo, Barbara ; Balzan, Stefania ; Novelli, Enrico ; Fontana, Federico ; Caburlotto, Greta ; Manfrin, Amedeo ; Fasolato, Luca</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-6962e130a834e52373420825091db503ab39bae26bb5a5f7b5e2f3e4bf76de803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>crustacean</topic><topic>emerging risks</topic><topic>MLSA</topic><topic>Vibrio spp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahman, Mohammad Shamsur</creatorcontrib><creatorcontrib>Currò, Sarah</creatorcontrib><creatorcontrib>Carraro, Lisa</creatorcontrib><creatorcontrib>Cardazzo, Barbara</creatorcontrib><creatorcontrib>Balzan, Stefania</creatorcontrib><creatorcontrib>Novelli, Enrico</creatorcontrib><creatorcontrib>Fontana, Federico</creatorcontrib><creatorcontrib>Caburlotto, Greta</creatorcontrib><creatorcontrib>Manfrin, Amedeo</creatorcontrib><creatorcontrib>Fasolato, Luca</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Italian journal of food safety</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahman, Mohammad Shamsur</au><au>Currò, Sarah</au><au>Carraro, Lisa</au><au>Cardazzo, Barbara</au><au>Balzan, Stefania</au><au>Novelli, Enrico</au><au>Fontana, Federico</au><au>Caburlotto, Greta</au><au>Manfrin, Amedeo</au><au>Fasolato, Luca</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Retrospective analysis of Vibrio spp. isolated from marketed crustaceans using multilocus sequence analysis</atitle><jtitle>Italian journal of food safety</jtitle><addtitle>Ital J Food Saf</addtitle><date>2023-03-08</date><risdate>2023</risdate><volume>12</volume><issue>1</issue><spage>11045</spage><epage>11045</epage><pages>11045-11045</pages><issn>2239-7132</issn><eissn>2239-7132</eissn><abstract>The genus
includes bacteria with different morphological and metabolic characteristics responsible for different human and animal diseases. An accurate identification is essential to assess the risks in regard to aquatic organisms and consequently to public health. The Multilocus Sequence Analysis (MLSA) scheme developed on the basis of 4 housekeeping genes (
B,
H,
A and
A) was applied to identify 92
strains isolated from crustaceans in 2011. Concatenated sequences were used for the phylogenetic and population analyses and the results were compared with those from biochemical identification tests. From the phylogenetic analysis, 10 clusters and 4 singletons emerged, whereas the population analysis highlighted 12 subpopulations that were well supported by phylogeny with few exceptions. The retrospective analysis allowed correct re-attribution of isolated species, indicating how, for some pathogens, there may be an overestimation of phenotypic identification (
.
). Use of the PubMLST
database highlighted a possible genetic link between Sequence Type (ST) 529 and ST195 (
) isolated from a human case in Norway during 2018. In addition to the identification of major risk groups of
,
and
, MLSA could be a valid support for species considered a minor risk, such as
,
and
. Due to the increased incidence of vibriosis in Europe, the application of different tools will also have to be considered to investigate the possible epidemiological links of the various species in the perspective of
to protect the consumer.</abstract><cop>Italy</cop><pub>PAGEPress Publications, Pavia, Italy</pub><pmid>37064522</pmid><doi>10.4081/ijfs.2023.11045</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-7746-1150</orcidid><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central |
| subjects | crustacean emerging risks MLSA Vibrio spp |
| title | Retrospective analysis of Vibrio spp. isolated from marketed crustaceans using multilocus sequence analysis |
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