Rapid Bladder Interleukin-10 Synthesis in Response to Uropathogenic Escherichia coli Is Part of a Defense Strategy Triggered by the Major Bacterial Flagellar Filament FliC and Contingent on TLR5

• Published in: mSphere 2019-11, Vol.4 (6)
• Main Authors: Acharya, Dhruba, Sullivan, Matthew J, Duell, Benjamin L, Goh, Kelvin G K, Katupitiya, Lahiru, Gosling, Dean, Chamoun, Michelle N, Kakkanat, Asha, Chattopadhyay, Debasish, Crowley, Michael, Crossman, David K, Schembri, Mark A, Ulett, Glen C
• Format: Article
• Language: English
• Subjects: Bacteria; Bladder; Chemokines; Cytokines; Disease; E coli; Epithelial cells; Escherichia coli; Experiments; Flagella; Gene amplification; Gene expression; Genomes; Host-Microbe Biology; Immune response; Innate immunity; Interleukin 1; Interleukin 10; Interleukin 6; Monocytes; Motility; Pathogenesis; Pathogens; Plasmids; Proteins; Ribonucleic acid; RNA; TLR5 protein; Toll-like receptors; Transcription; Tumor necrosis factor-TNF; Urinary tract; urinary tract infection; Urinary tract infections; Urogenital system; uropathogenic Escherichia coli
• ISSN: 2379-5042; 2379-5042
• DOI: 10.1128/mSphere.00545-19

Urinary tract infection (UTI) caused by uropathogenic (UPEC) engages interleukin-10 (IL-10) as an early innate immune response to regulate inflammation and promote the control of bladder infection. However, the mechanism of engagement of innate immunity by UPEC that leads to elicitation of IL-10 in the bladder is unknown. Here, we identify the major UPEC flagellar filament, FliC, as a key bacterial component sensed by the bladder innate immune system responsible for the induction of IL-10 synthesis. IL-10 responses of human as well as mouse bladder epithelial cell-monocyte cocultures were triggered by flagella of three major UPEC representative strains, CFT073, UTI89, and EC958. FliC purified to homogeneity induced IL-10 and as well as other functionally related cytokines, including IL-6. The genome-wide innate immunological context of FliC-induced IL-10 in the bladder was defined using RNA sequencing that revealed a network of transcriptional and antibacterial defenses comprising 1,400 genes that were induced by FliC. Of the FliC-responsive bladder transcriptome, altered expression of and 808 additional genes were dependent on Toll-like receptor 5 (TLR5), according to analysis of TLR5-deficient mice. Examination of the potential of FliC and associated innate immune signature in the bladder to boost host defense, based on prophylactic or therapeutic administration to mice, revealed significant benefits for the control of UPEC. We conclude that detection of FliC through TLR5 triggers rapid IL-10 synthesis in the bladder, and FliC represents a potential immune modulator that might offer benefit for the treatment or prevention of UPEC UTI. Interleukin-10 is part of the immune response to urinary tract infection (UTI) due to , and it is important in the early control of infection in the bladder. Defining the mechanism of engagement of the immune system by the bacteria that enables the protective IL-10 response is critical to exploring how we might exploit this mechanism for new infection control strategies. In this study, we reveal part of the bacterial flagellar apparatus (FliC) is an important component that is sensed by and responsible for induction of IL-10 in the response to UPEC. We show this response occurs in a TLR5-dependent manner. Using infection prevention and control trials in mice infected with , this study also provides evidence that purified FliC might be of value in novel approaches for the treatment of UTI or in preventing infection by exploi