Methanol Extract from Anogeissus leiocarpus (DC) Guill. et Perr. (Combretaceae) Stem Bark Quenches the Quorum Sensing of Pseudomonas aeruginosa PAO1

• Published in: Medicines (Basel, Switzerland) Switzerland), 2016-10, Vol.3 (4), p.26
• Main Authors: Ouedraogo, Vincent, Kiendrebeogo, Martin
• Format: Article
• Language: English
• Subjects: Anogeissus leiocarpus; Chromobacterium violaceum CV026; Pseudomonas aeruginosa PAO1; pyocyanin; quorum sensing
• ISSN: 2305-6320; 2305-6320
• DOI: 10.3390/medicines3040026

Due to its extensive arsenal of virulence factors and inherent resistance to antibiotics, is a threat particularly in immunocompromised patients. Considering the central role of quorum sensing in the production of virulence factors, inhibition of bacterial communication mechanism constitute an opportunity to attenuate pathogenicity of bacteria resistant to available antibiotics. Our study aimed to assess the anti-quorum sensing activity of , traditionally used in Burkina Faso, for the treatment of infected burn wounds. Investigations were carried out on methanol extract from stem bark. The reporter strains CV026 and PAO1 derivatives were used to evidence any interference with the bacterial quorum sensing and expression of related genes. PAO1 was used to measure the impact on pyocyanin production. At a sub-inhibitory concentration (100 µg/mL), methanol extract quenched the quorum sensing mechanism of PAO1 by down-streaming the gene, with a subsequent reduction of pyocyanin production. Moreover, the antioxidant polyphenols evidenced are able to reduce the oxidative stress induced by pyocyanin. The antioxidant and anti-quorum sensing activities of stem bark could justify its traditional use in the treatment of infected burn wounds.