Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity

Nanometer-scale properties of the extracellular matrix influence many biological processes, including cell motility. While much information is available for single-cell migration, to date, no knowledge exists on how the nanoscale presentation of extracellular matrix receptors influences collective c...

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Bibliographic Details
Published in:eLife 2021-09, Vol.10
Main Authors: Di Russo, Jacopo, Young, Jennifer L, Wegner, Julian WR, Steins, Timmy, Kessler, Horst, Spatz, Joachim P
Format: Article
Language:English
Subjects:
Adhesives
Cell adhesion & migration
Cell Biology
Cell migration
collective cell migration
Efficiency
Experiments
Extracellular matrix
Fibronectin
Hydrogels
integrin α5β1
Keratinocytes
keratocytes
Ligands
Motility
Physics of Living Systems
Velocity
Wound healing
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Summary:Nanometer-scale properties of the extracellular matrix influence many biological processes, including cell motility. While much information is available for single-cell migration, to date, no knowledge exists on how the nanoscale presentation of extracellular matrix receptors influences collective cell migration. In wound healing, basal keratinocytes collectively migrate on a fibronectin-rich provisional basement membrane to re-epithelialize the injured skin. Among other receptors, the fibronectin receptor integrin α5β1 plays a pivotal role in this process. Using a highly specific integrin α5β1 peptidomimetic combined with nanopatterned hydrogels, we show that keratinocyte sheets regulate their migration ability at an optimal integrin α5β1 nanospacing. This efficiency relies on the effective propagation of stresses within the cell monolayer independent of substrate stiffness. For the first time, this work highlights the importance of extracellular matrix receptor nanoscale organization required for efficient tissue regeneration.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.69861