Prolonged morphine administration alters protein expression in the rat myocardium

Morphine is used in clinical practice as a highly effective painkiller as well as the drug of choice for treatment of certain heart diseases. However, there is lack of information about its effect on protein expression in the heart. Therefore, here we aimed to identify the presumed alterations in ra...

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Bibliographic Details
Published in:Journal of biomedical science 2011-11, Vol.18 (1), p.89-89, Article 89
Main Authors: Drastichova, Zdenka, Skrabalova, Jitka, Neckar, Jan, Kolar, Frantisek, Novotny, Jiri
Format: Article
Language:English
Subjects:
Analgesics, Opioid - administration & dosage
Analgesics, Opioid - pharmacology
Animals
Clinical medicine
Drug dosages
Experiments
Laboratory animals
Male
morphine
Morphine - administration & dosage
Morphine - pharmacology
Myocardium - metabolism
Proteins
Proteins - metabolism
Proteomics
rat myocardium
Rats
Rats, Wistar
Rodents
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Summary:Morphine is used in clinical practice as a highly effective painkiller as well as the drug of choice for treatment of certain heart diseases. However, there is lack of information about its effect on protein expression in the heart. Therefore, here we aimed to identify the presumed alterations in rat myocardial protein levels after prolonged morphine treatment. Morphine was administered to adult male Wistar rats in high doses (10 mg/kg per day) for 10 days. Proteins from the plasma membrane- and mitochondria-enriched fractions or cytosolic proteins isolated from left ventricles were run on 2D gel electrophoresis, scanned and quantified with specific software to reveal differentially expressed proteins. Nine proteins were found to show markedly altered expression levels in samples from morphine-treaded rats and these proteins were identified by mass spectrometric analysis. They belong to different cell pathways including signaling, cytoprotective, and structural elements. The present identification of several important myocardial proteins altered by prolonged morphine treatment points to global effects of this drug on heart tissue. These findings represent an initial step toward a more complex view on the action of morphine on the heart.
ISSN:1423-0127
1021-7770
1423-0127
DOI:10.1186/1423-0127-18-89