Isolation and Typing of Streptococcus mutans and Streptococcus sobrinus from Caries-active Subjects

• Published in: Contemporary clinical dentistry 2017-10, Vol.8 (4), p.587-593
• Main Authors: Salman, Hamzah Abdulrahman, Senthilkumar, R, Imran, Khalid, Selvam, K Panneer
• Format: Article
• Language: English
• Subjects: Agar; Ampicillin; and filled teeth; Antibacterial agents; Antibiotics; Bacitracin; Biotypes; Biotyping; Care and treatment; Colonies; decay; Dental caries; Deoxyribonucleic acid; Disease; DNA; Dosage and administration; Erythromycin; Identification; Minimum inhibitory concentration; missing; morphotyping; mutans streptococci; Oral hygiene; Original; phylogenetic; Phylogenetics; Phylogeny; Population studies; Public health; rRNA 16S; Strains (organisms); Streptococcus; Streptococcus infections; Streptococcus mutans; Streptococcus sobrinus; Typing
• ISSN: 0976-237X; 0976-2361
• DOI: 10.4103/ccd.ccd_610_17

and are main etiological agents of dental caries. The aim of the study was to isolate, identify, characterize, and determine the minimum inhibitory concentration (MIC) of and from caries-active subjects. Sixty-five plaque samples were collected from caries-active subjects aged between 35 and 44 years, processed and cultured on mitis salivarius bacitracin agar. All the bacterial isolates were subjected to morphotyping and the suspected colonies were identified by 16S rDNA sequencing. The and strains were characterized by biotyping and phylogenetic analysis. The MIC of ampicillin and erythromycin was determined by microtiter plate method. Of the study population, 41 isolates displayed typical colony morphologies of and . The 16S rDNA sequencing results revealed that 36 isolates were and 5 isolates were . The biotyping of these isolates demonstrated three biotypes, namely, biotype I ( = 35), biotype III ( = 1), and biotype IV ( = 2). However, 3 isolates exhibited variant biotypes. The phylogenetic analysis revealed that the clinical strains of and clustered independently along with respective reference strains. The average MIC of ampicillin and erythromycin against and was 0.047 μg/ml and 0.39 μg/ml, respectively. The 16S rDNA sequencing was an impeccable method for and identification when compared with morphotyping and biotyping methods. The study also suggested that nonspecific bacteria might be involved in caries formation.