10-Pentadecenoic Acid Inhibits Melanogenesis Partly Through Suppressing the Intracellular MITF/Tyrosinase Axis

Melanogenesis, the biological process responsible for melanin synthesis, plays a crucial role in determining skin and hair color, photoprotection, and serving as a biomarker in various diseases. While various factors regulate melanogenesis, the role of fatty acids in this process remains underexplor...

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Bibliographic Details
Published in:Antioxidants 2024-12, Vol.13 (12), p.1547
Main Authors: Lee, Min-Kyeong, Moon, Kyoung Mi, Park, Su-Yeon, Seo, Jaeseong, Kim, Ah-Reum, Lee, Bonggi
Format: Article
Language:English
Subjects:
10(E)-pentadecenoic acid
Cytotoxicity
Enzymes
Fatty acids
Fibroblasts
Free radicals (Chemistry)
Intermedin
Intracellular
Kinases
Lipids
Melanin
Melanocyte-stimulating hormone
melanogenesis
Melanoma
Metabolism
Microphthalmia-associated transcription factor
MITF
Nuclear transport
Pigmentation
Proteins
RNA
Skin
Skin diseases
Skin pigmentation
tyrosinase
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Summary:Melanogenesis, the biological process responsible for melanin synthesis, plays a crucial role in determining skin and hair color, photoprotection, and serving as a biomarker in various diseases. While various factors regulate melanogenesis, the role of fatty acids in this process remains underexplored. This study investigated the anti-melanogenic properties of 10(E)-pentadecenoic acid (10E-PDA) through both in silico and in vitro analyses. SwissSimilarity was utilized to predict the functional properties of 10E-PDA by comparing it with structurally similar lipids known to exhibit anti-melanogenic effects. Subsequent in vitro experiments demonstrated that 10E-PDA significantly reduced melanin production and intracellular tyrosinase activity in α-MSH (melanocyte-stimulating hormone)-stimulated B16F10 melanoma cells without exhibiting significant cytotoxicity at concentrations up to 15 μM. Further mechanistic studies revealed that 10E-PDA inhibited the nuclear translocation of microphthalmia-associated transcription factor (MITF), consistent with the decrease observed in p-MITF protein levels. It also decreased the mRNA levels of tyrosinase-related proteins (TRP-1, TRP-2) and tyrosinase, while reducing the protein levels of TRP-1 and tyrosinase, but not TRP-2. These findings suggest that 10E-PDA exerts its anti-melanogenic effects by modulating the MITF/tyrosinase axis, presenting potential therapeutic implications for skin pigmentation disorders.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox13121547