Effect of prostaglandin analogs on matrix metalloproteinases and tissue inhibitor of metalloproteinases in eyelid muscle specimens

To characterize the effect of prostaglandin analogs (PAs) on tissue specific expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in levator aponeurosis resections (LAR) and conjunctiva-Muller muscle resections (CMMR). Specimens from LAR and CMMR of PA us...

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Bibliographic Details
Published in:Clinical ophthalmology (Auckland, N.Z.) N.Z.), 2018-01, Vol.12, p.2039-2046
Main Authors: Karli, Sapir, Ayala-Haedo, Juan Alfredo, Feuer, William J, Fernandez, Maria, Dubovy, Sander, Wester, Sara T
Format: Article
Language:English
Subjects:
Adipose tissue
Analysis
Bimatoprost
Collagen
Diabetic retinopathy
Glaucoma
Immunoglobulins
Immunohistochemistry
Kinases
Laboratories
matrix metalloproteinase
Medical records
MMP
Ophthalmology
Original Research
PAP
Pathology
periorbitopathy
prostaglandin analogue
prostaglandin associated periorbitopathy
Prostaglandins
Surgery
Travoprost
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Summary:To characterize the effect of prostaglandin analogs (PAs) on tissue specific expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in levator aponeurosis resections (LAR) and conjunctiva-Muller muscle resections (CMMR). Specimens from LAR and CMMR of PA users and non-users were analyzed for tissue specific expression of MMP-3, MMP-7, MMP-9 and TIMP-2 using immunohistochemistry. PA use, marginal reflex distances, levator function and palpebral fissure were documented through chart review. The associations between MMP expression, PA exposure time and ocular characteristics were evaluated with a two-factor analysis of variance and multiple correlation analysis. We observed a tissue specific pattern of expression of MMPs and TIMP-2 in relation to PA exposure between CMMR and LAR specimens. There was increased MMP-7 and TIMP-2 expression in muscle compared to collagen and adipose tissue ( ≤0.005), as well as a statistically significant difference in the relationship of MMP-3, MMP-9 and TIMP-2 levels to PA exposure in the two types of muscles (all ≤0.011). Adipose tissue had a PA-dependent reduced expression of MMP-3 (
ISSN:1177-5467
1177-5483
1177-5483
DOI:10.2147/opth.s178106