Metabolomics reveals altered biochemical phenotype of an invasive plant with potential to impair its biocontrol agent’s establishment and effectiveness

• Published in: Scientific reports 2024-11, Vol.14 (1), p.27150-16, Article 27150
• Main Authors: Barrett, D. Paul, Subbaraj, Arvind K., Pakeman, Robin J., Peterson, Paul, McCormick, Andrea Clavijo
• Format: Article
• Language: English
• Subjects: 631/158; 631/92; Animals; Biological control; Biological Control Agents - metabolism; Coleoptera - metabolism; Coleoptera - physiology; Humanities and Social Sciences; Introduced Species; Invasive plants; Invasive species; Life history; Metabolites; Metabolome; Metabolomics; Metabolomics - methods; multidisciplinary; New Zealand; Pest Control, Biological; Phenotype; Phenotypes; Phenotypic variations; Phenylpropanoids; Science; Science (multidisciplinary); Secondary metabolites; Soil nutrients; Ultraviolet radiation
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-76228-w

A plausible, but largely unexplored reason for many weed biocontrol agents failing to establish or being ineffective, could involve abiotically induced changes to an invasive plants’ biochemical phenotype and consequent enhanced herbivore resistance. Considerable literature demonstrates that chemically altered plant phenotypes can impair insect life history performance. Heather beetle, ( Lochmaea suturalis ), introduced to control invasive heather ( Calluna vulgaris ) in New Zealand (NZ) was difficult to establish and displays variable effectiveness. Using UHPLC-MS non-targeted metabolomics, we analysed primary and secondary metabolites of C. vulgaris from its native range (Scotland) and it’s introduced range (NZ), between which, differences in soil nutrients and ultraviolet light exist. We also explored secondary metabolite variation between sites within each range. New Zealand samples had the highest number of amplified metabolites, most notably defensive phenylpropanoids, supporting the concept of abiotically induced upregulation of key biosynthetic pathways. Analysis of secondary metabolite variation within each range revealed differences between sites but found little correlation of phenylpropanoid levels being influenced by variable soil nutrients. These results validate questions about the possibility of abiotically altered biochemical phenotypes in invasive plants, influencing weed biocontrol agent establishment and effectiveness, and show the potential for metabolomics in assisting future, or retrospectively analysing biological control programmes.