Isolation, characterization, and cross‐amplification of 20 microsatellite markers for Conospermum undulatum (Proteaceae)

Premise Recent habitat fragmentation is posing a risk to the wavy‐leaved smokebush, Conospermum undulatum (Proteaceae), a rare plant species endemic to southwestern Western Australia. Microsatellite markers are required to characterize the genetic diversity and structure of the species for conservat...

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Bibliographic Details
Published in:Applications in plant sciences 2019-08, Vol.7 (8), p.e11283-n/a
Main Authors: Delnevo, Nicola, Piotti, Andrea, Etten, Eddie J., Stock, William D., Byrne, Margaret
Format: Article
Language:English
Subjects:
Australia
Biodiversity
Conospermum undulatum
Conservation
Deoxyribonucleic acid
Design
Dispersal
DNA
Endemic species
Experiments
Genetic diversity
Genetic markers
Habitat fragmentation
Heterozygosity
Hybridization
microsatellite primers
Microsatellites
Plant sciences
Polymorphism
Population
Population genetics
Primer Note
Primer Notes
Proteaceae
Rare species
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Summary:Premise Recent habitat fragmentation is posing a risk to the wavy‐leaved smokebush, Conospermum undulatum (Proteaceae), a rare plant species endemic to southwestern Western Australia. Microsatellite markers are required to characterize the genetic diversity and structure of the species for conservation purposes and to facilitate ecological studies. Methods and Results Illumina MiSeq high‐throughput sequencing was used to develop 20 novel microsatellite markers for C. undulatum. Polymorphism at each locus was assessed using 72 individuals from three natural populations. Nineteen markers were polymorphic, with the number of alleles per locus ranging from two to 21, and observed and expected heterozygosity ranging from 0.000 to 1.000 and 0.117 to 0.919, respectively. All markers successfully amplified in three congeneric species (C. stoechadis, C. canaliculatum and C. triplinervium). Conclusions The microsatellite markers will be useful for revealing patterns of genetic diversity, dispersal dynamics, and hybridization events for C. undulatum to inform future conservation efforts.
ISSN:2168-0450
2168-0450
DOI:10.1002/aps3.11283