Peptide Specific Nanoplastic Detection Based on Sandwich Typed Localized Surface Plasmon Resonance

Recently, various waste microplastics sensors have been introduced in response to environmental and biological hazards posed by waste microplastics. In particular, the detrimental effects of nano-sized plastics or nanoplastics have been reported to be severe. Moreover, there have been many difficult...

Full description

Saved in:
Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2021-10, Vol.11 (11), p.2887
Main Authors: Oh, Seungju, Hur, Hyeyeon, Kim, Yoonjae, Shin, Seongcheol, Woo, Hyunjeong, Choi, Jonghoon, Lee, Hyun Ho
Format: Article
Language:English
Subjects:
Amino acids
Biohazards
Conjugation
Fourier transforms
Gold
LSPR
Methods
Microplastics
Morphology
Nanoparticles
nanoplastics
peptide binding
Peptides
Plastic pollution
Polystyrene
Polystyrene resins
Resonance
sandwich assay
Scanning electron microscopy
Sensors
Spectrum analysis
Styrofoam
Surface plasmon resonance
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recently, various waste microplastics sensors have been introduced in response to environmental and biological hazards posed by waste microplastics. In particular, the detrimental effects of nano-sized plastics or nanoplastics have been reported to be severe. Moreover, there have been many difficulties for sensing microplastics due to the limited methodologies for selectively recognizing nanoplastics. In this study, a customized gold nanoparticles (Au NPs) based localized surface plasmon resonance (LSPR) system having bio-mimicked peptide probes toward the nanoplastics was demonstrated. The specific determination through the oligo-peptide recognition was accomplished by chemical conjugation both on the LSPR chip's 40~50 nm Au NPs and sandwiched 5 nm Au NPs, respectively. The peptide probe could selectively bind to polystyrene (PS) nanoplastics in the forms of fragmented debris by cryo-grinding. A simple UV-Vis spectrophotometer was used to identify the LSPR sensing by primarily measuring the absorbance change and shift of absorption peak. The sandwich-binding could increase the LSPR detection sensitivity up to 60% due to consecutive plasmonic effects. In addition, microwave-boiled DI water inside of a styrofoam container was tested for putative PS nanoplastics resource as a real accessible sample. The LSPR system could be a novel protocol overcoming the limitations from conventional nanoplastic detection.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano11112887