Establishment of a Mouse Degenerative Model of Patellar Tendinopathy with Upregulation of Inflammation

There is no mouse model of patellar tendinopathy. This study aimed to establish a mouse inflammatory and degenerative patellar tendon injury model, which will facilitate research on patellar tendinopathy using advanced molecular tools including transgenic models. Collagenase at different doses (low...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-04, Vol.25 (7), p.3847
Main Authors: Lui, Pauline Po Yee, Liang, Zuru, Tan, Ri Min, Yung, Patrick Shu Hang
Format: Article
Language:English
Subjects:
animal model
Animals
Biomechanics
Calcification
Collagen
collagenase
Disease
Disease Models, Animal
Fibroblasts
Genetic engineering
Histology
Histopathology
Immunohistochemistry
Inflammation
Mice
Musculoskeletal Diseases
Pain
patellar tendinopathy
Pathogenesis
Tendinopathy
Tendon Injuries
Tendons
Up-Regulation
X-Ray Microtomography
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Summary:There is no mouse model of patellar tendinopathy. This study aimed to establish a mouse inflammatory and degenerative patellar tendon injury model, which will facilitate research on patellar tendinopathy using advanced molecular tools including transgenic models. Collagenase at different doses (low dose (LD), medium dose (MD), high dose (HD)) or saline was injected over the mouse patellar tendon. At weeks 1, 2, 4, and 8 post-injection, the tendons were harvested for histology and further examined by micro-computed tomography (microCT) imaging at week 8. The optimal dose group and the saline group were further evaluated by immunohistochemical staining, gait pattern, and biomechanical properties. The histopathological score increased dose-dependently post-collagenase injection. Ectopic mineralization was observed and increased with collagenase dose. The LD group was selected for further analysis. The expression of IL-10, TNF-α, and MMP-1 significantly increased post-injection. The changes of limb idleness index (ΔLII) compared to preinjury state were significantly higher, while the ultimate load, stiffness, ultimate stress, and maximum Young's modulus were significantly lower in the LD group compared to the saline group. A mouse inflammatory degenerative model of patellar tendon injury resembling tendinopathy was established as indicated by the dose-dependent increase in tendon histopathology, ectopic calcification, decrease in biomechanical properties, and pain-associated gait changes.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25073847