Staphylococcus aureus from Subclinical Cases of Mastitis in Dairy Cattle in Poland, What Are They Hiding? Antibiotic Resistance and Virulence Profile

Bovine mastitis is a common disease worldwide, and staphylococci are one of the most important etiological factors of this disease. show adaptability to new conditions, by which monitoring their virulence and antibiotic resistance mechanisms is extremely important, as it can lead to the development...

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Published in:Pathogens (Basel) 2022-11, Vol.11 (12), p.1404
Main Authors: Kaczorek-Łukowska, Edyta, Małaczewska, Joanna, Sowińska, Patrycja, Szymańska, Marta, Wójcik, Ewelina Agnieszka, Siwicki, Andrzej Krzysztof
Format: Article
Language:English
Subjects:
Adaptability
AMR
Antibiotic resistance
Antibiotics
Bacteria
biofilm
Biofilms
Cattle
Cefoxitin
Dairy cattle
Dairy industry
DNA fingerprinting
Drug resistance
Enzymes
Farms
Fingerprinting
Genes
Genotyping
Mastitis
Milk
Minimum inhibitory concentration
Oxacillin
Oxytetracycline
Pathogens
Penicillin
S. aureus
Staphylococcus aureus
Strains (organisms)
Vancomycin
Veterinary medicine
Virulence
virulence factors
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Summary:Bovine mastitis is a common disease worldwide, and staphylococci are one of the most important etiological factors of this disease. show adaptability to new conditions, by which monitoring their virulence and antibiotic resistance mechanisms is extremely important, as it can lead to the development of new therapies and prevention programs. In this study, we analyzed ( = 28) obtained from dairy cattle with subclinical mastitis in Poland. The sensitivity of the isolated strains to antibiotics were confirmed by the disc diffusion method. Additionally, minimum inhibitory concentration values were determined for vancomycin, cefoxitin and oxacillin. Genotyping was performed by two methods: PCR melting profile and MLVF-PCR (multiple-locus variable-number tandem-repeat fingerprinting). Furthermore, the presence of antibiotic resistance and virulence genes were checked using PCR reactions. The analyzed strains showed the greatest resistance to penicillin (57%), oxytetracycline (25%) and tetracycline (18%). Among the analyzed staphylococci, the presence of 9 of 15 selected virulence-related genes was confirmed, of which the , and genes were confirmed in all staphylococci. Biofilm was observed in the great majority of the analyzed bacteria (at least 70%). In the case of genotyping among the analyzed staphylococci (combined analysis of results from two methods), 14 patterns were distinguished, of which type 2 was the dominant one ( = 10). This study provides new data that highlights the importance of the dominance of biofilm over antibiotic resistance among the analyzed strains.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens11121404