Experimental Infection and Detection of Necrotizing Hepatopancreatitis Bacterium in the American Lobster Homarus americanus

Necrotizing hepatopancreatitis bacterium (NHPB) is an obligated intracellular bacteria causing severe hepatopancreatic damages and mass mortalities in penaeid shrimp. The worldwide distribution of penaeid shrimp as alien species threatens the life cycle of other crustacean species. The aim of the ex...

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Bibliographic Details
Published in:TheScientificWorld 2012-01, Vol.2012 (2012), p.1-4
Main Authors: Avila-Villa, Luz A., Gollas-Galván, Teresa, Martínez-Porchas, Marcel, Mendoza-Cano, Fernando, Hernández-López, Jorge
Format: Article
Language:English
Subjects:
American lobster
Animal Feed
Animals
Bacteria - metabolism
Bacterial Infections - metabolism
Contamination
Crustacea
Crustaceans
DNA - metabolism
Ecosystem
Experimental infection
Feces
Food contamination
Genome
Health aspects
Hepatopancreas
Hepatopancreas - microbiology
Homarus americanus
Identification and classification
Inoculation
Introduced species
Life cycle engineering
Life Cycle Stages
Life cycles
Litopenaeus vannamei
Lobsters
Necrosis
Nephropidae - microbiology
Penaeus
Polymerase chain reaction
Polymerase Chain Reaction - methods
Rickettsia
Species
Temperature
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Summary:Necrotizing hepatopancreatitis bacterium (NHPB) is an obligated intracellular bacteria causing severe hepatopancreatic damages and mass mortalities in penaeid shrimp. The worldwide distribution of penaeid shrimp as alien species threatens the life cycle of other crustacean species. The aim of the experiment was to evaluate the possibility of experimentally infecting the American lobster (Homarus americanus) with NHPB extracted from shrimp hepatopancreas. Homogenates from infected shrimp were fed by force to lobsters. Other group of lobsters was fed with homogenates of NHPB-free hepatopancreas. After the 15th day from initial inoculation, the presence of NHPB was detected by polymerase chain reaction in feces and hepatopancreas from lobsters inoculated with infected homogenates. Necrotized spots were observed in the surface of lobster hepatopancreas. In contrast, lobsters fed on NHPB-free homogenates resulted negative for NHPB. Evidence suggests the plasticity of NHPB which can infect crustacean from different species and inhabiting diverse latitudes. Considering the results, the American lobster could be a good candidate to maintain available NHPB in vivo.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1100/2012/979381