Jasmonate ZIM Domain Protein ( JAZ ) Gene SLJAZ15 Increases Resistance to Orobanche aegyptiaca in Tomato

Pers. is a holoparasitic plant that severely reduces tomato ( L.) production in China. However, there is a lack of effective control methods and few known sources of genetic resistance. In this study, we focused on key genes in the family, comparing the family in (L. Heynh.) to the tomato genome. Af...

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Bibliographic Details
Published in:Plants (Basel) 2024-05, Vol.13 (11), p.1493
Main Authors: Chen, Siyu, Zhang, Lu, Ma, Qianqian, Chen, Meixiu, Cao, Xiaolei, Zhao, Sifeng, Zhang, Xuekun
Format: Article
Language:English
Subjects:
Abiotic stress
Acids
Beans
Carotenoids
Chromosomes
Control
Control methods
Crop yields
Disease resistance
Diseases and pests
Environmental aspects
Enzymes
Gene amplification
Gene silencing
Genes
Genetic aspects
Heterologous overexpression
Homology
Identification and classification
Jasmonates
Jasmonic acid
JAZ family
Legumes
Linear analysis
Localization
Metabolism
Mimosaceae
Orobanche aegyptiaca
Parasitic plants
Phylogeny
Physiological aspects
Plant growth
Proteins
Signal transduction
SLJAZ15
Solanum lycopersicum
Tobacco
Tomatoes
VIGS
Viruses
Zinc finger proteins
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Summary:Pers. is a holoparasitic plant that severely reduces tomato ( L.) production in China. However, there is a lack of effective control methods and few known sources of genetic resistance. In this study, we focused on key genes in the family, comparing the family in (L. Heynh.) to the tomato genome. After identifying the family members in , we performed chromosomal localization and linear analysis with phylogenetic relationship analysis of the family. We also analyzed the gene structure of the gene family members in tomato and the homology of the genes among the different species to study their relatedness. The key genes for resistance were identified using VIGS (virus-induced gene silencing), and the parasitization rate of silenced tomato plants against increased by 47.23-91.13%. The genes were localized in the nucleus by subcellular localization. Heterologous overexpression in showed that the key gene had a strong effect on the parasitization process of , and the overexpression of the key gene reduced the parasitization rate of 1.69-fold. Finally, it was found that the gene can positively regulate the hormone content in tomato plants and affect plant growth and development, further elucidating the function of this gene.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants13111493